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Literature summary for 3.1.3.2 extracted from

  • Sowa, N.A.; Vadakkan, K.I.; Zylka, M.J.
    Recombinant mouse PAP has pH-dependent ectonucleotidase activity and acts through A(1)-adenosine receptors to mediate antinociception (2009), PLoS ONE, 4, e4248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged variant expressed in Hi5 insect cells using a baculovirus expression system Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
L-(+)-Tartrate
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q8CE08
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Mus musculus 4-nitrophenol + phosphate
-
 ?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
-
 Mus musculus 6,8-difluoro-4-methylumbelliferone + phosphate
-
 ?
AMP + H2O at neutral pH: to a lesser extent also ATP is dephosphorylated, at pH 5.6: also ADP, and to a lesser extent ATP are dephosphorylated Mus musculus adenosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
ectonucleotidase
-
 Mus musculus
prostatic acid phosphatase secretory isoform Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.45
-
-
 Mus musculus L-(+)-Tartrate