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Literature summary for 3.1.3.2 extracted from

  • Singh, H.; Felts, R.L.; Ma, L.; Malinski, T.J.; Calcutt, M.J.; Reilly, T.J.; Tanner, J.J.
    Expression, purification and crystallization of class C acid phosphatases from Francisella tularensis and Pasteurella multocida (2009), Acta Crystallogr. Sect. F, 65, 226-231.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Pasteurella multocida
His-tagged version expressed in Escherichia coli BL21(DE3) Francisella tularensis subsp. tularensis SCHU S4

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop method of vapor diffusion Francisella tularensis subsp. tularensis SCHU S4
sitting-drop method of vapor diffusion Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Francisella tularensis subsp. tularensis SCHU S4 Q5NIC0
-
-
Pasteurella multocida B9VWB2
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, immobilized metal ion affinity chromatography (Ni2+) Francisella tularensis subsp. tularensis SCHU S4
immobilized metal ion affinity chromatography (Ni2+), cation-exchange chromatography Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
 Francisella tularensis subsp. tularensis SCHU S4 p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pasteurella multocida p-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
class C acid phosphatase
-
 Francisella tularensis subsp. tularensis SCHU S4
class C acid phosphatase
-
 Pasteurella multocida