Activating Compound | Comment | Organism | Structure |
---|---|---|---|
6-ethylmercaptopurine | 1 mM, activity: 281%, isoenzyme 2 | Labeo rohita | |
6-ethylmercaptopurine | activity: 108% | Labeo rohita | |
adenine | 1 mM, activity: 194%, isoenzyme 2 | Labeo rohita | |
adenosine | 1 mM, activity: 134%, isoenzyme 2 | Labeo rohita | |
ADP | 1 mM, activity: 118%, isoenzyme 2 | Labeo rohita | |
AMP | 1 mM, activity: 104%, isoenzyme 1 | Labeo rohita | |
AMP | 1 mM, activity: 162%, isoenzyme 2 | Labeo rohita | |
cAMP | 1 mM, activity: 106%, isoenzyme 1 | Labeo rohita | |
cAMP | 1 mM, activity: 126%, isoenzyme 2 | Labeo rohita | |
cGMP | 1 mM, activity: 106%, isoenzyme 1 | Labeo rohita | |
cGMP | 1 mM, activity: 250%, isoenzyme 2 | Labeo rohita | |
GMP | 1 mM, activity: 104%, isoenzyme 1 | Labeo rohita | |
GMP | 1 mM, activity: 168%, isoenzyme 2 | Labeo rohita | |
guanine | 1 mM, activity: 107%, isoenzyme 2 | Labeo rohita | |
guanosine | 1 mM, activity: 299%, isoenzyme 2 | Labeo rohita |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
inorganic phosphate | - |
Labeo rohita | |
molybdate | - |
Labeo rohita | |
pyridoxal 5'-phosphate | - |
Labeo rohita | |
pyridoxamine 5'-phosphate | - |
Labeo rohita | |
Tartrate | - |
Labeo rohita | |
vanadate | - |
Labeo rohita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl phosphate | pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0093 | Labeo rohita | |
0.16 | - |
Phenyl phosphate | pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0076 | Labeo rohita | |
0.18 | - |
phosphotyrosine | pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0058 | Labeo rohita | |
0.2 | - |
flavin mononucleotide | pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0082 | Labeo rohita | |
0.23 | - |
4-nitrophenyl phosphate | pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.038 | Labeo rohita | |
0.31 | - |
Phenyl phosphate | pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.032 | Labeo rohita | |
0.41 | - |
flavin mononucleotide | pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0375 | Labeo rohita | |
1.1 | - |
phosphotyrosine | pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0154 | Labeo rohita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
isoenzyme 1, gel filtration | Labeo rohita |
18000 | - |
isoenzyme 2, gel filtration | Labeo rohita |
100000 | - |
gel filtration | Labeo rohita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Labeo rohita | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using gel filtration and affinity chromatography. Purification factor: 12.5 | Labeo rohita |
using gel filtration and affinity chromatography. Purification factor: 165 | Labeo rohita |
using gel filtration and affinity chromatography. Purification factor: 900 | Labeo rohita |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Labeo rohita | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
- |
Labeo rohita |
6.58 | - |
isoenzyme 1 | Labeo rohita |
35.6 | - |
isoenzyme 2 | Labeo rohita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | relative hydrolytic rate: 100% | Labeo rohita | 4-nitrophenol + phosphate | - |
? | |
adenosine monophosphate + H2O | relative hydrolytic rate: 50% | Labeo rohita | adenosine + phosphate | - |
? | |
adenosine triphosphate + H2O | relative hydrolytic rate: 25% | Labeo rohita | ATP + phosphate | - |
? | |
alpha-glycerophosphate + H2O | relative hydrolytic rate: 13% | Labeo rohita | glycerol + phosphate | - |
? | |
alpha-glycerophosphate + H2O | relative hydrolytic rate: 15% | Labeo rohita | glycerol + phosphate | - |
? | |
alpha-glycerophosphate + H2O | relative hydrolytic rate: 19% | Labeo rohita | glycerol + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | relative hydrolytic rate: 3% | Labeo rohita | 1-naphthol + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | relative hydrolytic rate: 8% | Labeo rohita | 1-naphthol + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | relative hydrolytic rate: 88% | Labeo rohita | 1-naphthol + phosphate | - |
? | |
beta-glycerophosphate + H2O | relative hydrolytic rate: 2% | Labeo rohita | glycerol + phosphate | - |
? | |
beta-glycerophosphate + H2O | relative hydrolytic rate: 70% | Labeo rohita | glycerol + phosphate | - |
? | |
beta-glycerophosphate + H2O | relative hydrolytic rate: 8% | Labeo rohita | glycerol + phosphate | - |
? | |
beta-naphthyl phosphate + H2O | relative hydrolytic rate: 116% | Labeo rohita | 2-naphthol + phosphate | - |
? | |
beta-naphthyl phosphate + H2O | relative hydrolytic rate: 70% | Labeo rohita | 2-naphthol + phosphate | - |
? | |
beta-naphthyl phosphate + H2O | relative hydrolytic rate: 81% | Labeo rohita | 2-naphthol + phosphate | - |
? | |
cytidine monophosphate + H2O | relative hydrolytic rate: 47% | Labeo rohita | cytidine + phosphate | - |
? | |
D-glucose-1-phosphate + H2O | relative hydrolytic rate: 13% | Labeo rohita | D-glucose + phosphate | - |
? | |
D-glucose-6-phosphate + H2O | relative hydrolytic rate: 14% | Labeo rohita | D-glucose + phosphate | - |
? | |
flavin mononucleotide + H2O | relative hydrolytic rate: 45% | Labeo rohita | riboflavin + phosphate | - |
? | |
flavin mononucleotide + H2O | relative hydrolytic rate: 91% | Labeo rohita | riboflavin + phosphate | - |
? | |
flavin mononucleotide + H2O | relative hydrolytic rate: 98% | Labeo rohita | riboflavin + phosphate | - |
? | |
guanosine monophosphate + H2O | relative hydrolytic rate: 32% | Labeo rohita | guanosine + phosphate | - |
? | |
phenyl phosphate + H2O | relative hydrolytic rate: 79% | Labeo rohita | phenol + phosphate | - |
? | |
phenyl phosphate + H2O | relative hydrolytic rate: 87% | Labeo rohita | phenol + phosphate | - |
? | |
phenyl phosphate + H2O | relative hydrolytic rate: 89% | Labeo rohita | phenol + phosphate | - |
? | |
phosphotyrosine + H2O | relative hydrolytic rate: 24% | Labeo rohita | tyrosine + phosphate | - |
? | |
phosphotyrosine + H2O | relative hydrolytic rate: 35% | Labeo rohita | tyrosine + phosphate | - |
? | |
phosphotyrosine + H2O | relative hydrolytic rate: 73% | Labeo rohita | tyrosine + phosphate | - |
? | |
uridine monophosphate + H2O | relative hydrolytic rate: 41% | Labeo rohita | uridine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18000, isoenzyme 1, SDS-PAGE | Labeo rohita |
monomer | 1 * 18000, isoenzyme 2, SDS-PAGE | Labeo rohita |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase | - |
Labeo rohita |
high molecular weight acid phosphatase | - |
Labeo rohita |
HM-ACP | - |
Labeo rohita |
IF1 | isoform 1 | Labeo rohita |
IF2 | isoform 2 | Labeo rohita |
LM-ACP | - |
Labeo rohita |
low molecular weight acid phosphatase | - |
Labeo rohita |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Labeo rohita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Labeo rohita |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
vanadate | - |
Labeo rohita | |
0.0009 | - |
molybdate | - |
Labeo rohita | |
0.013 | - |
pyridoxamine 5'-phosphate | pH 5.5, 37°C, isoenzyme 2 | Labeo rohita | |
0.017 | - |
pyridoxamine 5'-phosphate | pH 5.5, 37°C, isoenzyme 1 | Labeo rohita | |
0.02 | - |
pyridoxal 5'-phosphate | pH 5.5, 37°C, isoenzyme 2 | Labeo rohita | |
0.024 | - |
Tartrate | - |
Labeo rohita | |
0.025 | - |
vanadate | pH 5.5, 37°C, isoenzyme 2 | Labeo rohita | |
0.08 | - |
molybdate | pH 5.5, 37°C, isoenzyme 2 | Labeo rohita | |
0.138 | - |
vanadate | pH 5.5, 37°C, isoenzyme 1 | Labeo rohita | |
0.2 | - |
molybdate | pH 5.5, 37°C, isoenzyme 1 | Labeo rohita | |
0.208 | - |
pyridoxal 5'-phosphate | pH 5.5, 37°C, isoenzyme 1 | Labeo rohita | |
1.4 | - |
inorganic phosphate | pH 5.5, 37°C, isoenzyme 2 | Labeo rohita | |
1.5 | - |
inorganic phosphate | - |
Labeo rohita | |
2.7 | - |
inorganic phosphate | pH 5.5, 37°C, isoenzyme 1 | Labeo rohita |