Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 136.55% activity at 0.1% (w/v) | Arachis hypogaea | |
additional information | not affected by L-cysteine, tetradecyl-trimethyl-ammoniumbromide 0.1% (w/v) and hexadecyl-trimethyl-ammoniumbromide 0.1% (w/v) | Arachis hypogaea | |
Nonidet P 40 | 107.8% activity at 1% (w/v) | Arachis hypogaea | |
Nonidet P 40 | 146.5% activity at 1% (w/v) | Arachis hypogaea | |
Nonidet P 40 | 166.7% activity at 1% (w/v) | Arachis hypogaea | |
polyoxyethylene-9-lauryl ether | 120.4% activity at 1% (w/v) | Arachis hypogaea | |
polyoxyethylene-9-lauryl ether | 125% activity at 1% (w/v) | Arachis hypogaea | |
polyoxyethylene-9-lauryl ether | 147.4% activity at 1% (w/v) | Arachis hypogaea | |
taurocholic acid | 114% activity at 1% (w/v) | Arachis hypogaea | |
taurocholic acid | 136.3% activity at 1% (w/v) | Arachis hypogaea | |
taurocholic acid | 168.3% activity at 1% (w/v) | Arachis hypogaea | |
Triton X-100 | 124.5% activity at 1% (w/v) | Arachis hypogaea | |
Triton X-100 | 132.5% activity at 1% (w/v) | Arachis hypogaea | |
Triton X-100 | 169.6% activity at 1% (w/v) | Arachis hypogaea |
General Stability | Organism |
---|---|
stable in the presence of Na+ and K+ | Arachis hypogaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-2,2' dinitro-dibenzoate | 13.26% residual activity at 0.1% (w/v); 39.83% residual activity at 0.1% (w/v); 9.1% residual activity at 0.1% (w/v) | Arachis hypogaea | |
Ba2+ | 30.3% residual activity at 1 mM; 50.9% residual activity at 1 mM | Arachis hypogaea | |
beta-mercaptoethanol | 72.72% residual activity at 0.1% (w/v); 75.98% residual activity at 0.1% (w/v); 92.8% residual activity at 0.1% (w/v) | Arachis hypogaea | |
Ca2+ | 80.2% residual activity at 5 mM; 86.2% residual activity at 5 mM | Arachis hypogaea | |
Cu2+ | 25.7% residual activity at 5 mM; 30% residual activity at 5 mM; 64.8% residual activity at 1 mM | Arachis hypogaea | |
dithiothreitol | 64.34% activity at 0.1% (w/v); 98.7% residual activity at 0.1% (w/v) | Arachis hypogaea | |
EDTA | 40% residual activity at 5 mM; 85% residual activity at 5 mM; 92.2% residual activity at 5 mM | Arachis hypogaea | |
Fe3+ | 76% residual activity at 5 mM; 85% residual activity at 5 mM | Arachis hypogaea | |
hexadecyl-trimethyl-ammoniumbromide | 87.8% residual activity at 0.1% (w/v) | Arachis hypogaea | |
K+ | 92.6% residual activity at 1 mM; 93.4% residual activity at 1 mM | Arachis hypogaea | |
L-cysteine | 72.72% residual activity at 0.1% (w/v); 85.27% residual activity at 0.1% (w/v) | Arachis hypogaea | |
Mg2+ | 66.5% residual activity at 5 mM; 90.8% residual activity at 5 mM | Arachis hypogaea | |
p-chloromercuribenzoate | 62.99% residual activity at 0.1% (w/v); 69.39% residual activity at 0.1% (w/v); 72.81% residual activity at 0.1% (w/v) | Arachis hypogaea | |
SDS | 10% residual activity at 0.1% (w/v); 22% residual activity at 0.1% (w/v); 3% residual activity at 0.1% (w/v) | Arachis hypogaea | |
Sr2+ | 45.7% residual activity at 5 mM | Arachis hypogaea | |
tetradecyl-trimethyl-ammoniumbromide | 50.6% residual activity at 0.1% (w/v) | Arachis hypogaea | |
Zn2+ | 15% residual activity at 5 mM; 19% residual activity at 5 mM; 3.1% residual activity at 5 mM | Arachis hypogaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
Phenylphosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
0.49 | - |
p-nitrophenyl phosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
0.76 | - |
p-nitrophenyl phosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
0.79 | - |
p-nitrophenyl phosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
1.16 | - |
ATP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
1.31 | - |
Phenylphosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
1.48 | - |
ADP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
1.5 | 2 | Phenylphosphate | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
1.77 | - |
ATP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
2.88 | - |
ATP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
4.63 | - |
ADP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea | |
5.71 | - |
ADP | at 37°C in 100 mM sodium acetate buffer (pH 5.0) | Arachis hypogaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 115% activity at 5 mM | Arachis hypogaea | |
Ba2+ | 223.3% activity at 5 mM | Arachis hypogaea | |
Ca2+ | 244% activity at 1 mM | Arachis hypogaea | |
Cu2+ | 190% activity at 5 mM | Arachis hypogaea | |
Fe3+ | 186% activity at 1 mM | Arachis hypogaea | |
K+ | 124.9% activity at 1 mM | Arachis hypogaea | |
K+ | 127.3% activity at 5 mM | Arachis hypogaea | |
Mg2+ | 143.6% activity at 5 mM | Arachis hypogaea | |
Mg2+ | 197% activity at 1 mM | Arachis hypogaea | |
Na+ | 118% activity at 5 mM | Arachis hypogaea | |
Na+ | 125% activity at 5 mM | Arachis hypogaea | |
Sr2+ | 131% activity at 5 mM | Arachis hypogaea | |
Sr2+ | 193% activity at 5 mM | Arachis hypogaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
1 * 14000 + 1 * 12000, isozyme PI, gel filtration | Arachis hypogaea |
14000 | - |
1 * 14000 + 1 * 12000, isozyme PI, gel filtration | Arachis hypogaea |
22400 | - |
isozyme PIIa, SDS-PAGE | Arachis hypogaea |
24000 | - |
isozyme PIIb, SDS-PAGE | Arachis hypogaea |
25300 | - |
isozyme PI, SDS-PAGE | Arachis hypogaea |
26000 | - |
isozyme PI, gel filtration | Arachis hypogaea |
Organic Solvent | Comment | Organism |
---|---|---|
Nonidet P 40 | stable in the presence of non-ionic detergents such as Nonidet P 40 | Arachis hypogaea |
Triton X-100 | stable in the presence of non-ionic detergents such as Triton X-100 | Arachis hypogaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose CL-6B chromatography, CM-Sepharose CL-6B chromatography, Sephacryl S-200 HR gel filtration, and Phenyl-Sepharose HP column chromatography | Arachis hypogaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arachis hypogaea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
crude cell extract | Arachis hypogaea |
30.5 | - |
isozyme PIIa, after 49.5fold purification | Arachis hypogaea |
36 | - |
isozyme PIIb, after 58.2fold purification | Arachis hypogaea |
54.7 | - |
isozyme PI, after 92fold purification | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine-2',3'-cyclomonophosphate + H2O | 63% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
adenosine-2',3'-cyclomonophosphate + H2O | 9% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
ADP + H2O | 100.2% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | AMP + phosphate | - |
? | |
ADP + H2O | 103.4% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | AMP + phosphate | - |
? | |
ADP + H2O | 81.5% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | AMP + phosphate | - |
? | |
AMP + H2O | 25.2% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | adenosine + phosphate | - |
? | |
AMP + H2O | 25.5% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | adenosine + phosphate | - |
? | |
AMP + H2O | 51.5% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | adenosine + phosphate | - |
? | |
ATP + H2O | 116.7% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ADP + phosphate | - |
? | |
ATP + H2O | 76.9% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ADP + phosphate | - |
? | |
ATP + H2O | 80.7% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ADP + phosphate | - |
? | |
D-galactose 1-phosphate + H2O | 44.7% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | D-galactose + phosphate | - |
? | |
D-galactose 1-phosphate + H2O | 8.8% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | D-galactose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | 21.4% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 30.4% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | D-glucose + phosphate | - |
? | |
diphosphate + H2O | 56.1% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | 2 phosphate | - |
? | |
diphosphate + H2O | 66.35% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | 2 phosphate | - |
? | |
additional information | no activity with adenosine-2',3'-cyclomonophosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate | Arachis hypogaea | ? | - |
? | |
additional information | no activity with D-glucose 1-phosphate, D-glucose 6-phosphate, and sodium phytate | Arachis hypogaea | ? | - |
? | |
additional information | no activity with uridyl-5'-phosphogalactose, D-galactose 1-phosphate, and sodium phytate | Arachis hypogaea | ? | - |
? | |
NAD+ + H2O | 15.4% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
NAD+ + H2O | 16.5% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
NAD+ + H2O | 26.6% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
NADP+ + H2O | 28% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | NAD+ + phosphate | - |
? | |
NADP+ + H2O | 36% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | NAD+ + phosphate | - |
? | |
NADP+ + H2O | 61.7% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | NAD+ + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | 100% activity | Arachis hypogaea | p-nitrophenol + phosphate | - |
? | |
phenylphosphate + H2O | 85.2% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | phenol + phosphate | - |
? | |
phenylphosphate + H2O | 89% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | phenol + phosphate | - |
? | |
phenylphosphate + H2O | 93.1% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | phenol + phosphate | - |
? | |
phytate + H2O | 20.1% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | ? | - |
? | |
sodium diphosphate + H2O | 87% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | sodium phosphate + phosphate | - |
? | |
uridyl-5'-phosphogalactose + H2O | 21% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | uridyl-5'-galactose + phosphate | - |
? | |
uridyl-5'-phosphogalactose + H2O | 25.5% activity compared to p-nitrophenyl phosphate | Arachis hypogaea | uridyl-5'-galactose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 14000 + 1 * 12000, isozyme PI, gel filtration | Arachis hypogaea |
monomer | 1 * 22400, isozyme PIIa, SDS-PAGE | Arachis hypogaea |
monomer | 1 * 24000, isozyme PIIb, SDS-PAGE | Arachis hypogaea |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase PI | isozyme | Arachis hypogaea |
acid phosphatase PIIa | isozyme, exhibits phytasic activity | Arachis hypogaea |
acid phosphatase PIIb | isozyme | Arachis hypogaea |
orthophosphoric-monoester phosphohydrolase | - |
Arachis hypogaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Arachis hypogaea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 80 | above 55°C, the activity declines rapidly as the temperature increases, but the enzyme is not completely inactivated even at 80°C | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Arachis hypogaea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.6 | 5.6 | at 37°C | Arachis hypogaea |
5 | 5.6 | at 37°C | Arachis hypogaea |