Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with of 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.1-8.5 and 28-36% (w/v) PEG 4000 | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28510 | - |
full-length recombinant P4, mass spectrometry analysis | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Chelating HP column chromatography, Cu-affinity column chromatography, and HiTrap SP Sepharose column chromatography | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + H2O | - |
Haemophilus influenzae | pyruvate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipoprotein e | class C acid phosphatase | Haemophilus influenzae |
P4 | - |
Haemophilus influenzae |