Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | - |
Corylus avellana | |
phosphate | competitive product inhibition | Corylus avellana | |
vanadate | - |
Corylus avellana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of the 3 isozymes | Corylus avellana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81500 | - |
gel filtration | Corylus avellana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corylus avellana | - |
several isozymes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | all 3 isozymes from seeds | Corylus avellana |
Purification (Comment) | Organism |
---|---|
3 isozymes 75fold, 389fold, and 191fold from seed cotyledons to homogeneity by successive ammonoum sulfate fractionation, gel filtration, concanavalin A affinity chromatography, and cation- and anion exchange chromatography | Corylus avellana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Corylus avellana | - |
seed | dormant, hazelnut, several isozymes | Corylus avellana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.8 | - |
purified APase1 | Corylus avellana |
117 | - |
purified APase3 | Corylus avellana |
239 | - |
purified APase2 | Corylus avellana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | substrate only of isozyme APase2 | Corylus avellana | AMP + phosphate | - |
? | |
ATP + H2O | - |
Corylus avellana | ADP + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | - |
Corylus avellana | D-fructose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Corylus avellana | D-glucose + phosphate | - |
? | |
diphosphate + H2O | - |
Corylus avellana | 2 phosphate | - |
? | |
glycerophosphate + H2O | - |
Corylus avellana | glycerol + phosphate | - |
? | |
additional information | substrate specificity of isozymes APase1-3, no activity with AMP and phytic acid, all 3 isozymes do not show phytase activity, while APase 1 and 2 show significant phosphotyrosine phosphatase activity | Corylus avellana | ? | - |
? | |
O-phospho-L-serine + H2O | - |
Corylus avellana | L-serine + phosphate | - |
? | |
O-phospho-L-threonine + H2O | - |
Corylus avellana | L-threonine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APase | - |
Corylus avellana |
orthophosphoric-monoester phosphohydrolase | - |
Corylus avellana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
APase1 | Corylus avellana |
5.6 | - |
APase2 | Corylus avellana |
6 | - |
APase3 | Corylus avellana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corylus avellana | APase1 | - |
5.1 |
Corylus avellana | APase2 | - |
6.9 |
Corylus avellana | APase3 | - |
7.3 |