Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged wild-type and mutant APase in Escherichia coli strain BL21(DE3) | Glycine max |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged wild-type and mutant VSPalpha in Escherichia coli strain BL21(DE3) | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
S106D | site-directed mutagenesis of VSPalpha, the mutation increases the enzyme activity about 20fold with substrate GMP compared to the wild-type enzyme | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cAMP | - |
Glycine max | |
GMP | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
GMP | pH 6.0, 37°C, recombinant enzyme | Glycine max | |
8.8 | - |
4-nitrophenol phosphate | pH 6.0, 37°C, recombinant enzyme | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, recombinant enzyme, SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | VSPalpha functions as temporary storage reserve and as plant acid phosphatase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | O49855 | APase | - |
Glycine max | P10742 | VSPalpha, i.e. VSP25, fragment | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant APase 3.0fold from Escherichia coli strain BL21(DE3) by ammonium sulfate precipitation and ion exchange chromatography | Glycine max |
recombinant GST-tagged wild-type and mutant VSPalpha from Escherichia coli strain BL21(DE3) by ammonium sulfate precipitation and ion exchange chromatography, or by glutathione affinity chromatography | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | APase | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
purified recombinant enzyme, substrate phospho-L-tyrosine | Glycine max |
3.2 | - |
purified recombinant enzyme, substrate 4-nitrophenyl phosphate | Glycine max |
20 | - |
purified recombinant S106D mutant enzyme, substrate phospho-L-tyrosine | Glycine max |
22.6 | - |
purified recombinant enzyme, substrate GMP | Glycine max |
35 | - |
purified recombinant S106D mutant enzyme, substrate 4-nitrophenyl phosphate | Glycine max |
72.1 | - |
purified recombinant GST-tagged enzyme, substrate 4-nitrophenyl phosphate | Glycine max |
439 | - |
purified recombinant S106D mutant enzyme, substrate GMP | Glycine max |
845 | - |
purified recombinant detagged enzyme, substrate GMP | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Glycine max | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Glycine max | AMP + phosphate | - |
? | |
FMN + H2O | - |
Glycine max | flavin nucleoside + phosphate | - |
? | |
GMP + H2O | - |
Glycine max | guanosine + phosphate | - |
? | |
GMP + H2O | best substrate, recombinant enzyme | Glycine max | guanosine + phosphate | - |
? | |
additional information | VSPalpha functions as temporary storage reserve and as plant acid phosphatase | Glycine max | ? | - |
? | |
additional information | ADP, FMN, and triphosphate are no substrates | Glycine max | ? | - |
? | |
additional information | no activity with triphosphate | Glycine max | ? | - |
? | |
phospho-L-tyrosine + H2O | - |
Glycine max | L-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, recombinant enzyme, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
APase | - |
Glycine max |
More | VSPalpha belongs to the haloacid dehalogenase superfamily | Glycine max |
vegetative storage protein alpha | - |
Glycine max |
VSP25 | - |
Glycine max |
VSPalpha | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Glycine max |
6 | - |
assay at | Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
GMP | pH 6.0, 37°C, recombinant GST-tagged enzyme | Glycine max |