Literature summary for 3.1.3.2 extracted from

Calderone, V.; Forleo, C.; Benvenuti, M.; Thaller, M.C.; Rossolini, G.M.; Mangani, S.
A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases (2006), J. Mol. Biol., 355, 708-721.

Cloned(Commentary)

Cloned (Comment)	Organism
gene aphA, overexpression in strain DH5alpha	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant AphA in complex with adenosine and phosphate, with dCMP, or with osmate, sitting drop method, 0.002 ml protein solution containing 10 mg/ml protein in sodium acetate buffer, pH 7.0, mixed with 0.1 ml of precipitant solution containing 17-22% w/v PEG 6000, and 1 mM MgCl2, 20°C, equilibration against 2 ml of precipitant solution, soaking in ligand solution containing 50 mM sodium acetate, pH 7.0, 35% w/v PEG 6000, and 50 mM ligand for ligand complexing, derivatizing with Au and spermine, X-ray diffraction structure determination and analysis at 1.25-2.14 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant AphA in complex with adenosine and phosphate, with dCMP, or with osmate, sitting drop method, 0.002 ml protein solution containing 10 mg/ml protein in sodium acetate buffer, pH 7.0, mixed with 0.1 ml of precipitant solution containing 17-22% w/v PEG 6000, and 1 mM MgCl2, 20°C, equilibration against 2 ml of precipitant solution, soaking in ligand solution containing 50 mM sodium acetate, pH 7.0, 35% w/v PEG 6000, and 50 mM ligand for ligand complexing, derivatizing with Au and spermine, X-ray diffraction structure determination and analysis at 1.25-2.14 A resolution

Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Escherichia coli	-	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AE22	gene aphA, strain MG1655	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant AphA from strain DH5alpha	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate	detailed structure-based catalytic mechanism	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + H2O	-	Escherichia coli	adenosine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
AphA	-	Escherichia coli
More	the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases	Escherichia coli