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Literature summary for 3.1.3.2 extracted from
- Acid metallophosphatase from the ameba Amoeba proteus (2004), J. Evol. Biochem. Physiol., 40, 28-36.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | reversible | Amoeba proteus |  |
| EDTA | reversible | Amoeba proteus | |
| EGTA | only partly reversible | Amoeba proteus | |
| Fe2+ | complete inhibition at 5-50 mM FeSO4 or Fe2(SO4)3 | Amoeba proteus | |
| Fe3+ | slight inhibition | Amoeba proteus | |
| additional information | the enzyme is tartrate-resistant | Amoeba proteus | |
| Zn2+ | inhibits 2 of 3 isozymes | Amoeba proteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates all 3 isozymes | Amoeba proteus | |
| Mg2+ | activates all 3 isozymes | Amoeba proteus | |
| additional information | no effect by Li+ | Amoeba proteus | |
| Zn2+ | the enzyme is a Zn-metallophosphatase containing an essential zinc ion in the active site | Amoeba proteus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amoeba proteus | - |
3 isozymes | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | the enzyme is a Zn-metallophosphatase containing an essential zinc ion in the active site | Amoeba proteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.203 | - |
substrate 1-naphthyl phosphate | Amoeba proteus |
| 2.712 | - |
substrate 4-nitrophenyl phosphate | Amoeba proteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | - |
Amoeba proteus | 1-naphthol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Amoeba proteus | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tartrate-resistant acid phosphatase | - |
Amoeba proteus |
| TR-AcPh | - |
Amoeba proteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Amoeba proteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at | Amoeba proteus |
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