Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tartrate | slight activation | Phaseolus vulgaris |
Cloned (Comment) | Organism |
---|---|
gene KeACP, DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
molybdate | complete inhibition at 0.1 mM | Phaseolus vulgaris | |
vanadate | 30 min, 30°C, leads to complete loss of phosphatase activity of the enzyme at 0.1 mM and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by citrate, EDTA, fluoride, Zn2+, Fe3+, and Mn2+ | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
56000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
96000 | - |
gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | Q764C1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | both subunit types are glycosylated | Phaseolus vulgaris |
Purification (Comment) | Organism |
---|---|
852fold to homogeneity from embryonic axes by DEAE ion exchange and concanavalin A affinity chromatography | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | expression during elongation in germination | Phaseolus vulgaris | - |
seed | embryonic axes, expression during elongation in germination | Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
383.3 | - |
purified enzyme | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
3-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Phaseolus vulgaris | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | low activity | Phaseolus vulgaris | AMP + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | alpha-naphthol + phosphate | - |
? | |
AMP + H2O | - |
Phaseolus vulgaris | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Phaseolus vulgaris | ADP + phosphate | - |
? | |
beta-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | beta-naphthol + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
additional information | substrate specificity, overview, vanadate leads to loss of phosphatase activity of the enzyme and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris | ? | - |
? | |
phospho-L-serine + H2O | low activity | Phaseolus vulgaris | L-serine + phosphate | - |
? | |
phospho-L-threonine + H2O | - |
Phaseolus vulgaris | L-threonine + phosphate | - |
? | |
phospho-L-tyrosine + H2O | - |
Phaseolus vulgaris | L-tyrosine + phosphate | - |
? | |
phosphoenolpyruvate + H2O | - |
Phaseolus vulgaris | pyruvate + phosphate | - |
? | |
phytate + H2O | low activity | Phaseolus vulgaris | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
More | three combinations of the two subunit types occur with the 56 kDa subunit dominating, no intersubunit disulfide linkages | Phaseolus vulgaris |
Synonyms | Comment | Organism |
---|---|---|
colorless acid phosphatase | - |
Phaseolus vulgaris |
KeACP | - |
Phaseolus vulgaris |
More | the colorless acid phosphatase is distinct from the purple acid phosphatase | Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Phaseolus vulgaris |