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Literature summary for 3.1.3.2 extracted from
- Purification and properties of an acid phosphatase from Entamoeba histolytica HM-1:IMSS (2000), Int. J. Parasitol., 30, 585-591.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.1 mM, slight activation of membrane-bound acid phosphatase to 110% of control | Entamoeba histolytica |  |
| okadaic acid | 0.001 mM, activation of membrane-bound acid phosphatase to 112% of control | Entamoeba histolytica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ammonium molybdate | 0.1 mM, 54% inhibition of secreted acid phosphatase, 96% inhibition of membrane-bound acid phosphatase | Entamoeba histolytica | |
| EDTA | 0.1 mM, 40% inhibition of secreted acid phosphatase | Entamoeba histolytica | |
| NaF | 0.1 mM, 70% inhibition of secreted acid phosphatase, 58% inhibition of membrane-bound acid phosphatase | Entamoeba histolytica | |
| sodium orthovanadate | 0.1 mM, 46% inhibition of secreted acid phosphatase, 91% inhibition of membrane-bound acid phosphatase | Entamoeba histolytica | |
| sodium tartrate | 0.1 mM, 41% inhibition of secreted acid phosphatase, 33% inhibition of membrane-bound acid phosphatase | Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
4-nitrophenyl phosphate | pH 5.5, 37°C | Entamoeba histolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | MAP, membrane-bound acid phosphatase | Entamoeba histolytica | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
x * 97000, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
HM-1:IMSS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.012 | - |
- |
Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Entamoeba histolytica | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 97000, SDS-PAGE | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Entamoeba histolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | about 40% of maximal activity at pH 4.5 and pH 6.5 | Entamoeba histolytica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | isoelectric focusing, pH gradient 3-10 | - |
5.5 |
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