Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 10 mM, 88% inhibition | Solanum lycopersicum | |
F- | - |
Solanum lycopersicum | |
FeCl3 | 10 mM, complete inhibition | Solanum lycopersicum | |
molybdate | - |
Solanum lycopersicum | |
phosphate | - |
Solanum lycopersicum | |
vanadate | - |
Solanum lycopersicum | |
ZnCl2 | 10 mM, 99% inhibition | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
GTP | pH 5.1, 25°C | Solanum lycopersicum | |
0.18 | - |
2-naphthyl phosphate | pH 5.1, 25°C | Solanum lycopersicum | |
0.22 | - |
phosphoenolpyruvate | pH 5.1, 25°C | Solanum lycopersicum | |
0.23 | - |
IDP | pH 5.1, 25°C | Solanum lycopersicum | |
0.5 | - |
ATP | pH 5.1, 25°C | Solanum lycopersicum | |
1.4 | - |
ADP | pH 5.1, 25°C | Solanum lycopersicum | |
1.4 | - |
bis-(p-nitrophenyl)phosphate | pH 5.1, 25°C | Solanum lycopersicum | |
2.5 | - |
UTP | pH 5.1, 25°C | Solanum lycopersicum | |
2.7 | - |
pyridoxal 5'-phosphate | - |
Solanum lycopersicum | |
3.4 | - |
diphosphate | pH 5.1, 25°C | Solanum lycopersicum | |
3.4 | - |
1-naphthyl phosphate | pH 5.1, 25°C | Solanum lycopersicum | |
3.6 | - |
GTP | pH 5.1, 25°C | Solanum lycopersicum | |
4.1 | - |
phosphotyrosine | pH 5.1, 25°C | Solanum lycopersicum | |
7.1 | - |
Phenyl phosphate | pH 5.1, 25°C | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Solanum lycopersicum | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 10 mM, 150% activated | Solanum lycopersicum | |
MnCl2 | 10 mM, 130% activated | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE | Solanum lycopersicum |
63000 | - |
alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE | Solanum lycopersicum |
142000 | - |
non-denaturing PAGE, gel filtration | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
- |
Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
620 | - |
- |
Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme is stable for at least 4 months | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Solanum lycopersicum | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | - |
Solanum lycopersicum | 2-naphthol + phosphate | - |
? | |
ADP + H2O | - |
Solanum lycopersicum | ? | - |
? | |
ATP + H2O | - |
Solanum lycopersicum | ? | - |
? | |
bis-(p-nitrophenyl)phosphate + H2O | - |
Solanum lycopersicum | ? | - |
? | |
diphosphate + H2O | - |
Solanum lycopersicum | 2 phosphate | - |
? | |
GDP + H2O | - |
Solanum lycopersicum | ? | - |
? | |
GTP + H2O | - |
Solanum lycopersicum | ? | - |
? | |
IDP + H2O | - |
Solanum lycopersicum | ? | - |
? | |
additional information | enzyme also exhibits significant peroxidase activity, which is optimal at pH 9.0 and insensitive to Mg2+ or molybdate | Solanum lycopersicum | ? | - |
? | |
phenyl phosphate + H2O | - |
Solanum lycopersicum | phenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | - |
Solanum lycopersicum | pyruvate + phosphate | - |
? | |
phosphotyrosine + H2O | - |
Solanum lycopersicum | ? | - |
? | |
pyridoxal 5'-phosphate + H2O | - |
Solanum lycopersicum | pyridoxal + phosphate | - |
? | |
UTP + H2O | - |
Solanum lycopersicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
IAP | - |
Solanum lycopersicum |
intracellular acid phosphatase | - |
Solanum lycopersicum |
purple acid phosphatase | - |
Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, 20% loss of activity | Solanum lycopersicum |
65 | - |
5 min, 50% loss of activity | Solanum lycopersicum |
70 | - |
5 min, 80% loss of activity | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
- |
Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
molybdate | pH 5.1 | Solanum lycopersicum | |
0.0004 | - |
molybdate | pH 5.7 | Solanum lycopersicum | |
0.0005 | - |
molybdate | pH 6.2 | Solanum lycopersicum | |
0.0065 | - |
vanadate | pH 5.1 | Solanum lycopersicum | |
0.013 | - |
vanadate | pH 5.7 | Solanum lycopersicum | |
0.015 | - |
vanadate | pH 6.2 | Solanum lycopersicum | |
0.094 | - |
F- | pH 5.1 | Solanum lycopersicum | |
0.14 | - |
phosphate | pH 5.7 | Solanum lycopersicum | |
0.14 | - |
F- | pH 5.7 | Solanum lycopersicum | |
0.42 | - |
phosphate | pH 5.7 | Solanum lycopersicum | |
0.85 | - |
F- | pH 6.2 | Solanum lycopersicum | |
1.6 | - |
phosphate | pH 5.1 | Solanum lycopersicum |