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Literature summary for 3.1.3.2 extracted from

  • Schenk, G.; Korsinczky, M.L.J.; Hume, D.A.; Hamilton, S.; DeJersey, J.
    Purple acid phosphatases from bacteria: similarities to mammalian and plant enzymes (2000), Gene, 255, 419-424.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 10 mM, 88% inhibition Solanum lycopersicum
F-
-
 Solanum lycopersicum
FeCl3 10 mM, complete inhibition Solanum lycopersicum
molybdate
-
 Solanum lycopersicum
phosphate
-
 Solanum lycopersicum
vanadate
-
 Solanum lycopersicum
ZnCl2 10 mM, 99% inhibition Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 GTP pH 5.1, 25°C Solanum lycopersicum
0.18
-
 2-naphthyl phosphate pH 5.1, 25°C Solanum lycopersicum
0.22
-
 phosphoenolpyruvate pH 5.1, 25°C Solanum lycopersicum
0.23
-
 IDP pH 5.1, 25°C Solanum lycopersicum
0.5
-
 ATP pH 5.1, 25°C Solanum lycopersicum
1.4
-
 ADP pH 5.1, 25°C Solanum lycopersicum
1.4
-
 bis-(p-nitrophenyl)phosphate pH 5.1, 25°C Solanum lycopersicum
2.5
-
 UTP pH 5.1, 25°C Solanum lycopersicum
2.7
-
 pyridoxal 5'-phosphate
-
 Solanum lycopersicum
3.4
-
 diphosphate pH 5.1, 25°C Solanum lycopersicum
3.4
-
 1-naphthyl phosphate pH 5.1, 25°C Solanum lycopersicum
3.6
-
 GTP pH 5.1, 25°C Solanum lycopersicum
4.1
-
 phosphotyrosine pH 5.1, 25°C Solanum lycopersicum
7.1
-
 Phenyl phosphate pH 5.1, 25°C Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Solanum lycopersicum 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 10 mM, 150% activated Solanum lycopersicum
MnCl2 10 mM, 130% activated Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum
63000
-
 alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum
142000
-
 non-denaturing PAGE, gel filtration Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
620
-
-
 Solanum lycopersicum

Storage Stability

Storage Stability Organism
-80°C, purified enzyme is stable for at least 4 months Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
 Solanum lycopersicum 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O
-
 Solanum lycopersicum 2-naphthol + phosphate
-
 ?
ADP + H2O
-
 Solanum lycopersicum ?
-
 ?
ATP + H2O
-
 Solanum lycopersicum ?
-
 ?
bis-(p-nitrophenyl)phosphate + H2O
-
 Solanum lycopersicum ?
-
 ?
diphosphate + H2O
-
 Solanum lycopersicum 2 phosphate
-
 ?
GDP + H2O
-
 Solanum lycopersicum ?
-
 ?
GTP + H2O
-
 Solanum lycopersicum ?
-
 ?
IDP + H2O
-
 Solanum lycopersicum ?
-
 ?
additional information enzyme also exhibits significant peroxidase activity, which is optimal at pH 9.0 and insensitive to Mg2+ or molybdate Solanum lycopersicum ?
-
 ?
phenyl phosphate + H2O
-
 Solanum lycopersicum phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Solanum lycopersicum pyruvate + phosphate
-
 ?
phosphotyrosine + H2O
-
 Solanum lycopersicum ?
-
 ?
pyridoxal 5'-phosphate + H2O
-
 Solanum lycopersicum pyridoxal + phosphate
-
 ?
UTP + H2O
-
 Solanum lycopersicum ?
-
 ?

Subunits

Subunits Comment Organism
dimer alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
IAP
-
 Solanum lycopersicum
intracellular acid phosphatase
-
 Solanum lycopersicum
purple acid phosphatase
-
 Solanum lycopersicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, 20% loss of activity Solanum lycopersicum
65
-
 5 min, 50% loss of activity Solanum lycopersicum
70
-
 5 min, 80% loss of activity Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
 Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 molybdate pH 5.1 Solanum lycopersicum
0.0004
-
 molybdate pH 5.7 Solanum lycopersicum
0.0005
-
 molybdate pH 6.2 Solanum lycopersicum
0.0065
-
 vanadate pH 5.1 Solanum lycopersicum
0.013
-
 vanadate pH 5.7 Solanum lycopersicum
0.015
-
 vanadate pH 6.2 Solanum lycopersicum
0.094
-
 F- pH 5.1 Solanum lycopersicum
0.14
-
 phosphate pH 5.7 Solanum lycopersicum
0.14
-
 F- pH 5.7 Solanum lycopersicum
0.42
-
 phosphate pH 5.7 Solanum lycopersicum
0.85
-
 F- pH 6.2 Solanum lycopersicum
1.6
-
 phosphate pH 5.1 Solanum lycopersicum