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Literature summary for 3.1.3.2 extracted from

  • Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
    Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures (2002), Eur. J. Biochem., 269, 6278-6286.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, SAP2, 50% inhibition Solanum lycopersicum
Ca2+ 5 mM, SAP2, 60% inhibition Solanum lycopersicum
Co2+ 5 mM, SAP2, 95% inhibition Solanum lycopersicum
Cu2+ 5 mM, SAP1, 91% inhibition Solanum lycopersicum
EDTA 5 mM, SAP2, 71% inhibition Solanum lycopersicum
F-
-
 Solanum lycopersicum
Mn2+ 5 mM, SAP2, 26% inhibition Solanum lycopersicum
molybdate competitive Solanum lycopersicum
additional information SAP1 and SAP2 are not inhibited by tartrate Solanum lycopersicum
phosphate mixed and competitive Solanum lycopersicum
Zn2+ 5 mM, SAP2, complete inhibition of SAP1 and SAP2 Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 phosphoenolpyruvate pH 5.3, 25°C, SAP2 Solanum lycopersicum
1.9
-
 Tetrapolyphosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
2.1
-
 phosphoenolpyruvate pH 5.3, 25°C, SAP1 Solanum lycopersicum
2.1
-
 Phenyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
2.1
-
 phosphotyrosine pH 5.3, 25°C, SAP2 Solanum lycopersicum
2.9
-
 Tetrapolyphosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.3
-
 4-nitrophenyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.9
-
 2-naphthyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
4.1
-
 ATP pH 5.3, 25°C, SAP1 Solanum lycopersicum
4.5
-
 6-phospho-D-gluconate
-
 Solanum lycopersicum
4.5
-
 4-nitrophenyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
4.5
-
 2-naphthyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
4.6
-
 diphosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
5
-
 glycerol 3-phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
5.3
-
 Phenyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
5.3
-
 1-naphthyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
5.4
-
 GTP pH 5.3, 25°C, SAP2 Solanum lycopersicum
5.5
-
 ATP pH 5.3, 25°C, SAP2 Solanum lycopersicum
6
-
 3-phosphoglycerate pH 5.3, 25°C, SAP2 Solanum lycopersicum
6.4
-
 1-naphthyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
8.6
-
 GTP pH 5.3, 25°C, SAP1 Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Solanum lycopersicum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, SAP1 is activated to 115% of control Solanum lycopersicum
EDTA 5 mM, SAP1 is activated to 114% of control Solanum lycopersicum
Mg2+ 5 mM, SAP1 is activated to 136% of control, SAP2 is activated to 181% of control Solanum lycopersicum
Mn2+ 5 mM, SAP1 is activated to 113% of control Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
two secreted acid phosphatases: SAP1 and SAP2 Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
246
-
 SAP1 Solanum lycopersicum
940
-
 SAP2 Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 2-naphthol + phosphate
-
 ?
3-phosphoglycerate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 4-nitrophenol + phosphate
-
 ?
6-phospho-D-gluconate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum D-gluconate + phosphate
-
 ?
ATP + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?
diphosphate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum 2 phosphate
-
 ?
glycerol 3-phosphate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum glycerol + phosphate
-
 ?
GTP + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?
additional information SAP1 and SAP2 are multifunctional enzymes, the enzymes also exhibit significant peroxidase activity which is optimal at approximately pH 8.4 and insensitive to Mg2+ or molybdate Solanum lycopersicum ?
-
 ?
phenyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum pyruvate + phosphate
-
 ?
phosphotyrosine + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum tyrosine + phosphate
-
 ?
tetrapolyphosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?

Synonyms

Synonyms Comment Organism
purple acid phosphatase
-
 Solanum lycopersicum
SAP1
-
 Solanum lycopersicum
SAP2
-
 Solanum lycopersicum
secreted acid phosphatase
-
 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
 isoenzyme SAP1 and SAP2 Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 molybdate pH 5.3, 25°C, SAP1 or SAP2 Solanum lycopersicum
0.48
-
 F- pH 5.3, 25°C, SAP1 Solanum lycopersicum
0.95
-
 F- pH 5.3, 25°C, SAP1 Solanum lycopersicum
1.2
-
 phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
1.3
-
 phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum