Cloned (Comment) | Organism |
---|---|
- |
Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.194 | - |
4-nitrophenyl phosphate | pH 4.8, 37°C | Sinorhizobium meliloti | |
1.06 | - |
Alpha-naphthyl phosphate | pH 4.8, 37°C | Sinorhizobium meliloti | |
2.59 | - |
Phenyl phosphate | pH 4.8, 37°C | Sinorhizobium meliloti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Sinorhizobium meliloti | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35800 | - |
2 * 35800, SDS-PAGE | Sinorhizobium meliloti |
70000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Sinorhizobium meliloti 104A14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Sinorhizobium meliloti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Sinorhizobium meliloti | 1-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + phosphate | - |
? | |
AMP + H2O | - |
Sinorhizobium meliloti | adenosine + phosphate | - |
? | |
AMP + H2O | - |
Sinorhizobium meliloti 104A14 | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Sinorhizobium meliloti | ? | - |
? | |
ATP + H2O | - |
Sinorhizobium meliloti 104A14 | ? | - |
? | |
bis-p-nitrophenyl phosphate + H2O | - |
Sinorhizobium meliloti | p-nitrophenol + p-nitrophenyl phosphate | - |
? | |
D-ribose 5-phosphate + H2O | - |
Sinorhizobium meliloti | D-ribose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | - |
Sinorhizobium meliloti 104A14 | D-ribose + phosphate | - |
? | |
DL-myo-inositol-1-monophosphate + H2O | - |
Sinorhizobium meliloti | DL-myo-inositol + phosphate | - |
? | |
additional information | no activity detected with: DL-myo-inositol-hexaphosphate, D-glucose 6-phosphate, O-phospho-DL-serine, O-phospho-DL-tyrosine and O-phospho-DL-threonine | Sinorhizobium meliloti | ? | - |
? | |
additional information | no activity detected with: DL-myo-inositol-hexaphosphate, D-glucose 6-phosphate, O-phospho-DL-serine, O-phospho-DL-tyrosine and O-phospho-DL-threonine | Sinorhizobium meliloti 104A14 | ? | - |
? | |
phenolphthalein diphosphate + H2O | - |
Sinorhizobium meliloti | ? | - |
? | |
phenyl phosphate + H2O | - |
Sinorhizobium meliloti | phenol + phosphate | - |
? | |
phenyl phosphate + H2O | - |
Sinorhizobium meliloti 104A14 | phenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35800, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
NapE | - |
Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | reaction with 4-nitrophenyl phosphate | Sinorhizobium meliloti |
6.5 | - |
reaction with phenyl phosphate | Sinorhizobium meliloti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | pH 2.0: about 25% of maximal activity, pH 2.5: 65% of maximal activity, pH 6.0: about 40% of maximal activity, reaction with 4-nitrophenyl phosphate | Sinorhizobium meliloti |
6 | 7 | pH 6.0: about 40% of maximal activity, pH 7.0: about 35% of maximal activity, reaction with phenyl phosphate | Sinorhizobium meliloti |