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Literature summary for 3.1.3.2 extracted from
- Purification, enzymatic properties, and active site environment of a novel manganese(III)-containing acid phosphatase (1981), J. Biol. Chem., 256, 10664-10670.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Ipomoea batatas |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acetylacetone | - |
Ipomoea batatas |  |
| AsO43- | - |
Ipomoea batatas | |
| Bromosuccinimide | - |
Ipomoea batatas | |
| Cu2+ | - |
Ipomoea batatas | |
| F- | - |
Ipomoea batatas | |
| Hg2+ | - |
Ipomoea batatas | |
| MoO42- | - |
Ipomoea batatas | |
| PCMB | - |
Ipomoea batatas | |
| phosphate | - |
Ipomoea batatas | |
| Pyridine | - |
Ipomoea batatas | |
| Zn2+ | - |
Ipomoea batatas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn3+ | enzyme contains one atom of Mn(III) per 110000 Da enzyme | Ipomoea batatas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Ipomoea batatas |
| 110000 | - |
gel filtration | Ipomoea batatas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ipomoea batatas | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ipomoea batatas |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Ipomoea batatas | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ipomoea batatas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | 6% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | adenosine + phosphate | - |
? | |
| 2-glycerophosphate + H2O | 69% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | glycerol + phosphate | - |
? | |
| 3'-AMP + H2O | 60% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | adenosine + phosphate | - |
? | |
| 5'-ADP + H2O | 15% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | AMP + phosphate | - |
? | |
| 5'-AMP + H2O | 3% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | adenosine + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | 48% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | glycerol + phosphate | - |
? | |
| AMP + H2O | - |
Ipomoea batatas | adenosine + phosphate | - |
? | |
| ATP + H2O | 38% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | ADP + phosphate | - |
? | |
| D-fructose 1,6-diphosphate + H2O | 69% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | ? | - |
? | |
| D-fructose 6-phosphate + H2O | 29% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | D-fructose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | 25% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | D-ribose + phosphate | - |
? | |
| diphosphate + H2O | 93% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | 2 phosphate | - |
? | |
| FMN + H2O | 35% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | ? | - |
? | |
| Glucose 6-phosphate + H2O | 33% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | Glucose + phosphate | - |
? | |
| NADP+ + H2O | 68% of the activity with p-nitrophenyl phosphate | Ipomoea batatas | NAD+ + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Ipomoea batatas | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Ipomoea batatas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | isoenzyme PI-II and PII-I | Ipomoea batatas |
| 45 | 50 | isoenzyme PI-I | Ipomoea batatas |
| 45 | 60 | isoenzyme PII-II | Ipomoea batatas |
| 50 | 60 | isoenzyme PI-III and PIII-I | Ipomoea batatas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5.5 | isoenzyme PII-II | Ipomoea batatas |
| 5.5 | - |
- |
Ipomoea batatas |
| 5.8 | - |
- |
Ipomoea batatas |
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