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Literature summary for 3.1.3.10 extracted from
- Acid phosphatases of Escherichia coli: molecular cloning and analysis of agp, the structural gene for a periplasmic acid glucose phosphatase (1988), J. Bacteriol., 170, 4916.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vivo cloning technique with plasmid Mu dII4042, expression in Escherichia coli strain SBS1295 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
D-glucose 1-phosphate | - |
Escherichia coli |  |
| 15 | - |
D-glucose 6-phosphate | - |
Escherichia coli | |
| 25 | - |
D-fructose 6-phosphate | - |
Escherichia coli | |
| 30 | - |
4-nitrophenyl phosphate | - |
Escherichia coli | |
| 30 | - |
D-ribose 5-phosphate | - |
Escherichia coli | |
| 50 | - |
D-Fructose 1-phosphate | - |
Escherichia coli | |
| 100 | - |
Glycerol 2-phosphate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | periplasm extracts made by osmotic shock of strain SBS1405(pEP1376) | Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-Page of osmotic shock fluid of strain SBS1405(pEP1376) | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | Escherichia coli | - |
D-fructose + phosphate | - |
? | |
| D-fructose 1-phosphate + H2O | Escherichia coli | - |
D-fructose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | Escherichia coli | - |
D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | Escherichia coli | - |
D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | Escherichia coli | - |
D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | Escherichia coli | - |
D-ribose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? | |
| D-fructose 1,6-diphosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| D-fructose 1-phosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Escherichia coli | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Escherichia coli | D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | - |
Escherichia coli | D-ribose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Escherichia coli | glycerol + phosphate | - |
? | |
| glycerol 3-phosphate + H2O | - |
Escherichia coli | glycerol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 44000 + 1 * 44000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
temperature used in the assay with 25 mM 4-nitrophenyl phosphate in 50 mM potassium phthalate-HCl buffer pH 4.5 | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | with 4-nitrophenyl phosphate 25 mM as substrate | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | with 4-nitrophenyl-phosphate 25 mM as substrate | Escherichia coli |
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