Literature summary for 3.1.3.1 extracted from

Tibbitts, T.T.; Xu, X.; Kantrowitz, E.R.
Kinetics and crystal structure of a mutant Escherichia coli alkaline phosphatase (Asp-369->Asn): a mechanism involving one zinc per active site (1994), Protein Sci., 3, 2005-2014.

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Protein Variants

Protein Variants	Comment	Organism
D369A	mutant enzyme shows reduced turnover rates and increased Km-value	Escherichia coli
D369N	mutant enzyme shows reduced turnover rates and increased Km-value. The reaction mechanism of the mutant enzyme involves only 1 metal with the possible assistance of a His side chain	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0094	-	p-nitrophenyl phosphate	wild type enzyme, in absence of a phosphate acceptor	Escherichia coli
0.02124	-	p-nitrophenyl phosphate	wild type enzyme, in presence of a phosphate acceptor	Escherichia coli
0.05972	-	p-nitrophenyl phosphate	mutant enzyme D369N, in presence of a phosphate acceptor	Escherichia coli
0.176	-	p-nitrophenyl phosphate	mutant enzyme D369N, in absence of a phosphate acceptor	Escherichia coli
2.941	-	p-nitrophenyl phosphate	mutant enzyme D369A, in absence of a phosphate acceptor	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	p-nitrophenyl phosphate	mutant enzyme D369A, in absence of a phosphate acceptor	Escherichia coli
0.4	-	p-nitrophenyl phosphate	mutant enzyme D369N, in absence of a phosphate acceptor	Escherichia coli
44.5	-	p-nitrophenyl phosphate	wild type enzyme	Escherichia coli
80.5	-	p-nitrophenyl phosphate	wild type enzyme, in presence of a phosphate acceptor	Escherichia coli
230	-	p-nitrophenyl phosphate	mutant enzyme D369N, in presence of a phosphate acceptor	Escherichia coli

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Release 2023.1 (January 2023)