Protein Variants | Comment | Organism |
---|---|---|
D369A | mutant enzyme shows reduced turnover rates and increased Km-value | Escherichia coli |
D369N | mutant enzyme shows reduced turnover rates and increased Km-value. The reaction mechanism of the mutant enzyme involves only 1 metal with the possible assistance of a His side chain | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
p-nitrophenyl phosphate | wild type enzyme, in absence of a phosphate acceptor | Escherichia coli | |
0.02124 | - |
p-nitrophenyl phosphate | wild type enzyme, in presence of a phosphate acceptor | Escherichia coli | |
0.05972 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in presence of a phosphate acceptor | Escherichia coli | |
0.176 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in absence of a phosphate acceptor | Escherichia coli | |
2.941 | - |
p-nitrophenyl phosphate | mutant enzyme D369A, in absence of a phosphate acceptor | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
p-nitrophenyl phosphate | mutant enzyme D369A, in absence of a phosphate acceptor | Escherichia coli | |
0.4 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in absence of a phosphate acceptor | Escherichia coli | |
44.5 | - |
p-nitrophenyl phosphate | wild type enzyme | Escherichia coli | |
80.5 | - |
p-nitrophenyl phosphate | wild type enzyme, in presence of a phosphate acceptor | Escherichia coli | |
230 | - |
p-nitrophenyl phosphate | mutant enzyme D369N, in presence of a phosphate acceptor | Escherichia coli |