Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Kobori, H.; Taga, N.
    Extracellular alkaline phosphatase from marine bacteria: purification and properties of extracellular phosphatase from a marine Pseudomonas sp. (1980), Can. J. Microbiol., 26, 833-838.
No PubMed abstract available
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
anions required for maximal activity in the order of decreasing effectiveness: Cl-, Br-, NO3-, ClO4-, SCN- Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cys
-
 Pseudomonas sp.
EDTA
-
 Homo sapiens
EDTA inhibition is reversed by divalent cations Pseudomonas sp.
Hg2+ 1 mM, 82% loss of activity Pseudomonas sp.
KCN
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
192
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
5°C, pH 8.0, Tris-HCl buffer, in seawater, 10 days, 50% inactivation Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phosphoglycerate + H2O
-
 Homo sapiens glycerol + phosphate
-
 ?
2-phosphoglycerate + H2O
-
 Pseudomonas sp. glycerol + phosphate
-
 ?
ADP + H2O
-
 Homo sapiens ?
-
 ?
ADP + H2O
-
 Pseudomonas sp. ?
-
 ?
AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
AMP + H2O
-
 Pseudomonas sp. adenosine + phosphate
-
 ?
ATP + H2O
-
 Homo sapiens ?
-
 ?
ATP + H2O
-
 Pseudomonas sp. ?
-
 ?
diphosphate + H2O no activity Pseudomonas sp. 2 phosphate
-
 ?
glucose 1-phosphate + H2O
-
 Homo sapiens glucose + phosphate
-
 ?
glucose 1-phosphate + H2O
-
 Pseudomonas sp. glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O
-
 Pseudomonas sp. Glucose + phosphate
-
 ?
GMP + H2O
-
 Pseudomonas sp. guanosine + phosphate
-
 ?
GTP + H2O
-
 Pseudomonas sp. ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pseudomonas sp. p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
 Homo sapiens
50
-
 10 min, partial inactivation Pseudomonas sp.
75
-
 10 min, complete inactivation Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Homo sapiens