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Literature summary for 3.1.3.1 extracted from

  • Yedlin, S.T.; Young, G.P.; Seetharam, B.; Seetharam, S.; Alpers, D.H.
    Characterization and comparison of soluble and membranous forms of intestinal alkaline phosphatase from the suckling rat (1981), J. Biol. Chem., 256, 5620-5626.
    View publication on PubMed
Search for more literature for 3.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
Phe 20 mM, 82-99% inhibition of the soluble enzyme form, 68-95% inhibition of the particulate enzyme form Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 beta-Glycerophosphate soluble enzyme form Rattus norvegicus
0.38
-
 beta-Glycerophosphate membranous enzyme form Rattus norvegicus
0.39
-
 ATP membranous enzyme form Rattus norvegicus
0.44
-
 p-nitrophenyl phosphate membranous enzyme form Rattus norvegicus
0.45
-
 p-nitrophenyl phosphate soluble enzyme form Rattus norvegicus
0.48
-
 ATP soluble enzyme form Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, soluble enzyme form, SDS-PAGE Rattus norvegicus
56000
-
 2 * 56000, membranous enzyme form, SDS-PAGE Rattus norvegicus
95000
-
 soluble enzyme form, gel filtration Rattus norvegicus
98000
-
 membranous enzyme form, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein, the soluble form contains more fucose and N-acetylgalactosamine than does the membranous form. The soluble enzyme form contains 19.8% carbohydrate, the membranous enzyme form contains 16% carbohydrate Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
intestine the soluble and the membranous form are closely related but distinct isoenzymes Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
147
-
 membranous isoenzyme Rattus norvegicus
341
-
 soluble isoenzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phosphoglycerate + H2O
-
 Rattus norvegicus glycerol + phosphate
-
 ?
ATP + H2O
-
 Rattus norvegicus ?
-
 ?
beta-glycerophosphate + H2O
-
 Rattus norvegicus glycerol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, membranous enzyme form, SDS-PAGE Rattus norvegicus
dimer 2 * 47000, soluble enzyme form, SDS-PAGE Rattus norvegicus