General Stability | Organism |
---|---|
at 3.5 mM Ca2+, the enzyme is unstable at low NaCl concentrations but its stability increases with the salt concentration. At 32 mM Ca2+ , the enzyme is rather stable even at low NaCl concentrations. Even at 4 M NaCl, the stability of the enzyme depends on the Ca2+ concentration | Haloarcula marismortui |
both calcium ions (in the millimolar range) and NaCl (in the molar range) are required for the stability of the enzyme | Haloarcula marismortui |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Haloarcula marismortui |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted during phosphate starvation | Haloarcula marismortui | - |
- |
membrane | the enzyme is secreted during phosphate starvation | Haloarcula marismortui | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requires Ca2+ and not Zn2+ ions for its activity.Optimal Ca2+ concentration for enzymatic activity is 3.4 mM | Haloarcula marismortui | |
KCl | the enzyme is more active in NaCl than with KCl | Haloarcula marismortui | |
NaCl | required, the enzyme is more active in NaCl than with KCl | Haloarcula marismortui |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme appears as a broad peak at a location corresponding to molecules having molecular masses of 650 to 850 kDa. The native form of the enzyme is heterogeneous and composed of multiple oligomeric forms | Haloarcula marismortui |
160000 | - |
x * 160000, SDS-PAGE, the native form of the enzyme is heterogeneous and composed of multiple oligomeric forms | Haloarcula marismortui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Haloarcula marismortui |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | the enzyme is secreted during phosphate starvation | Haloarcula marismortui | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Haloarcula marismortui | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 160000, SDS-PAGE, the native form of the enzyme is heterogeneous and composed of multiple oligomeric forms | Haloarcula marismortui |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Haloarcula marismortui |
9 | - |
assay at | Haloarcula marismortui |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | pH 8.0: about 60% of maximal activity, pH 9.5: about 75% of maximal activity | Haloarcula marismortui |
Organism | Comment | Expression |
---|---|---|
Haloarcula marismortui | the enzyme is produced only when the cells are starved of phosphate. No activity can be detected when the phosphate concentration is higher than 0.1 mM, and the highest specific activity is obtained when the minimal medium is completely devoid of phosphate. When the cells are starved of phosphate, the production of the enzyme starts immediately and continues at a constant rate for at least 48 h | up |