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Literature summary for 3.1.3.1 extracted from

  • Marhuenda-Egea, F.C.; Piera-Velazquez, S.; Cadenas, C.; Cadenas, E.
    Kinetic regulation of an alkaline p-nitrophenylphosphate phosphatase from Halobacterium salinarum in low water system by Mn2+ and monovalent cations (2001), FEMS Microbiol. Lett., 198, 111-115.
    View publication on PubMed
Search for more literature for 3.1.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 384 mM NaCl, without Mn2+ Halobacterium salinarum
4.9
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 680 mM NaCl, without Mn2+ Halobacterium salinarum
8.6
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 680 mM NaCl, 2.5 mM Mn2+ Halobacterium salinarum
9
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 384 mM K+, 2.5 mM MnCl2 Halobacterium salinarum
9.7
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 680 mM K+, 2.5 mM MnCl2 Halobacterium salinarum
11.6
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 384 mM NaCl, 2.5 mM Mn2+ Halobacterium salinarum
13.9
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 192 mM NaCl, 2.5 mM Mn2+ Halobacterium salinarum
14.4
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 680 mM NaCl, 5 mM Mn2+ Halobacterium salinarum
16.7
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 192 mM NaCl, without Mn2+ Halobacterium salinarum
16.8
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 680 mM NaCl, 8 mM Mn2+ Halobacterium salinarum
25.4
-
 4-nitrophenyl phosphate pH 8.5, 40°C, enzyme in reversed micelles, 192 mM K+, 2.5 mM MnCl2 Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the kinetic behaviour is determined by the ratio between concentration of Mn2+ and concentration of Na+ or K+. When the Mn2+ increases and Na+ or K+ decreases, the kinetics show cooperative behaviour Halobacterium salinarum
Mn2+ the kinetic behaviour is determined by the ratio between concentration of Mn2+ and concentration of Na+ or K+. When the Mn2+ increases and Na+ or K+ decreases, the kinetics show cooperative behaviour Halobacterium salinarum
Na+ the kinetic behaviour is determined by the ratio between concentration of Mn2+ and concentration of Na+ or K+. When the Mn2+ increases and Na+ or K+ decreases, the kinetics show cooperative behaviour Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
-
-
Halobacterium salinarum NRC 36014
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Halobacterium salinarum 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Halobacterium salinarum NRC 36014 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline p-nitrophenylphosphate phosphatase
-
 Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Halobacterium salinarum