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Literature summary for 3.1.3.1 extracted from

  • Col, M.; Colak, A.; Ertunga, N.; Yildirim, M.; Özel, A.
    Cloning, expression and characterization of alkaline phosphatase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4 (2010), Turk. J. Biochem., 35, 208-214.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Parageobacillus caldoxylosilyticus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Parageobacillus caldoxylosilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087
-
4-nitrophenyl phosphate pH 9.5, 50°C, Vmax: 0.049 micromol/min/mg Parageobacillus caldoxylosilyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ inhibitory effect Parageobacillus caldoxylosilyticus
Cd2+ inhibitory effect Parageobacillus caldoxylosilyticus
Hg2+ inhibitory effect Parageobacillus caldoxylosilyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Parageobacillus caldoxylosilyticus
51590
-
calculated from cDNA Parageobacillus caldoxylosilyticus

Organism

Organism UniProt Comment Textmining
Parageobacillus caldoxylosilyticus C1K6P2
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Parageobacillus caldoxylosilyticus 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Parageobacillus caldoxylosilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 30 100% active for 2 h Parageobacillus caldoxylosilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Parageobacillus caldoxylosilyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
100% active after 12 h incubation at 4°C Parageobacillus caldoxylosilyticus