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Literature summary for 3.1.3.1 extracted from

  • Sekiguchi, S.; Hashida, Y.; Yasukawa, K.; Inouye, K.
    Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity (2011), Enzyme Microb. Technol., 49, 171-176.
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
diethanolamine highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine Bos taurus
N-Methylethanolamine highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine Bos taurus
triethanolamine highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 4-nitrophenyl phosphate the Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine Bos taurus
0.07
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M diethanolamine Bos taurus
0.09
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M methylamine Bos taurus
0.18
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.2 M N-methylethanolamine Bos taurus
0.24
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.1 M triethanolamine Bos taurus
0.3
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M ethanolamine Bos taurus
0.3
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M ethanolamine Bos taurus
0.31
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M triethanolamine Bos taurus
0.35
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.02 M dimethylamine Bos taurus
0.35
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.02 M ethylamine Bos taurus
0.43
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M ethylamine Bos taurus
0.62
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M methylamine Bos taurus
0.65
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M dimethylamine Bos taurus
0.83
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M diethanolamine Bos taurus
2.5 3 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M N-methylethanolamine Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Bos taurus 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Bos taurus
BIALP
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M methylamine Bos taurus
150
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.02 M dimethylamine Bos taurus
190
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M diethanolamine Bos taurus
300
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M ethanolamine Bos taurus
330
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M methylamine Bos taurus
370
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M dimethylamine Bos taurus
400
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M ethylamine Bos taurus
450
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.02 M ethylamine Bos taurus
500
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.2 M N-methylethanolamine Bos taurus
610
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.05 M ethanolamine Bos taurus
840
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 0.1 M triethanolamine Bos taurus
1240
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M diethanolamine Bos taurus
1450
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M triethanolamine Bos taurus
2250
-
 4-nitrophenyl phosphate pH 9.8, 20°C, 1 M N-methylethanolamine Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
 assay at Bos taurus