Literature summary for 3.1.3.1 extracted from

Luo, M.; Guo, Y.; Deng, J.; Wei, H.; Zhang, Z.; Leng, Y.; Men, D.; Song, L.; Zhang, X.; Zhou, Y.
Characterization of a monomeric heat-labile classical alkaline phosphatase from Anabaena sp. PCC7120 (2010), Biochemistry, 75, 655-664.

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Protein Variants

Protein Variants	Comment	Organism
D25A	mutant exhibits a slight increase in activity	Anabaena sp.
D460A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
D505A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
E445A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
H216A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
H464A	mutant is almost inactive in 1 M Tris buffer, but has an increased activity in 0.1 M Mops buffer	Anabaena sp.
H506A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
H566A	mutant is almost inactive in 1 M Tris buffer, but has an increased activity in 0.1 M Mops buffer	Anabaena sp.
R229A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
S165A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.
T218A	activity is significantly decreased in the presence or absence of a phosphate acceptor	Anabaena sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Anabaena sp.
phosphate	competitive inhibitor	Anabaena sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0145	-	4-nitrophenyl phosphate	pH 8, 25°C, in the presence of phosphate acceptor (1 M Tris-HCl buffer)	Anabaena sp.
3.6	-	4-nitrophenyl phosphate	pH 8, 25°C, without phosphate acceptor	Anabaena sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	enzyme exhibits 1.4fold activation in the presenece of 1 mM Co2+	Anabaena sp.
Mg2+	metalloenzyme containing Mg2+ and Zn2+ with a molar ratio of 1:2, enzyme exhibits 1.6fold activation in the presenece of 1 mM Co2+	Anabaena sp.
Zn2+	metalloenzyme containing Mg2+ and Zn2+ with a molar ratio of 1:2	Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	SDS-PAGE	Anabaena sp.
70000	-	gel filtration	Anabaena sp.

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp.	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6	-	pH 8, 25°C, without phosphate acceptor	Anabaena sp.
16.3	-	pH 8, 25°C, in the presence of phosphate acceptor (1 M Tris-HCl buffer)	Anabaena sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Anabaena sp.	4-nitrophenol + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Anabaena sp.

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase	-	Anabaena sp.
all2843	-	Anabaena sp.
alr5291	alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs	Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Anabaena sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	enzyme rapidly inactivated at 50°C	Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
29.9	-	4-nitrophenyl phosphate	pH 8, 25°C, in the presence of phosphate acceptor (1 M Tris-HCl buffer)	Anabaena sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Anabaena sp.
11	-	optimal pH	Anabaena sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
1	2	enzyme inactivated above pH 12	Anabaena sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0472	-	phosphate	pH 8.0, 25°C	Anabaena sp.

Expression

Organism	Comment	Expression
Anabaena sp.	transcription is upregulated during phosphate starving	up

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Release 2023.1 (January 2023)