Literature summary for 3.1.3.1 extracted from

Liu, X.; Wang, H.; Liang, Y.; Yang, J.; Zhang, H.; Lei, H.; Shen, Y.; Sun, Y.
Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol (2010), Mol. Biotechnol., 45, 56-64.

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Cloned(Commentary)

Cloned (Comment)	Organism
single-chain Fv antibody fusion protein expressed in Escherichia coli BL21(DE3)pLysS	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02982	-	4-nitrophenyl phosphate	single-chain Fv antibody fusion protein	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00634	gene phoA	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography	Escherichia coli

Renatured (Commentary)

Renatured (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Escherichia coli	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
113.6	-	4-nitrophenyl phosphate	single-chain Fv antibody fusion protein	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3810	-	4-nitrophenyl phosphate	single-chain Fv antibody fusion protein	Escherichia coli

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Release 2023.1 (January 2023)