Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Antarctic bacterium TAB5 |
Protein Variants | Comment | Organism |
---|---|---|
G149D | mutant shows increased activity after thermal inactivation at 60°C, 120 min: 11.4%, kcat (1/sec) (4-nitrophenyl phosphate): 117 | Antarctic bacterium TAB5 |
G87A | mutant shows increased activity after thermal inactivation at 60°C, 120 min: 11.4%, kcat (1/sec) (4-nitrophenyl phosphate): 100 | Antarctic bacterium TAB5 |
H135E | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 0.4%, kcat (1/sec) (4-nitrophenyl phosphate): 303 | Antarctic bacterium TAB5 |
H135E/G149D | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 1%, kcat (1/sec) (4-nitrophenyl phosphate): 361 | Antarctic bacterium TAB5 |
S338T | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 0.6%, kcat (1/sec) (4-nitrophenyl phosphate): 114 | Antarctic bacterium TAB5 |
S42G | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 0%, kcat (1/sec) (4-nitrophenyl phosphate): 133 | Antarctic bacterium TAB5 |
S42G/H135E | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 0%, kcat (1/sec) (4-nitrophenyl phosphate): 70 | Antarctic bacterium TAB5 |
S42G/S338T | double mutant shows decreased activity after thermal inactivation at 60°C, 120 min: 0%, kcat (1/sec) (4-nitrophenyl phosphate): 71 | Antarctic bacterium TAB5 |
S86A | mutant shows increased activity after thermal inactivation at 60°C, 120 min: 15.6%, kcat (1/sec) (4-nitrophenyl phosphate): 203 | Antarctic bacterium TAB5 |
S86A/G87A | double mutant shows increased activity after thermal inactivation at 60°C, 120 min: 39.4%, kcat (1/sec) (4-nitrophenyl phosphate): 30 | Antarctic bacterium TAB5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35860 | - |
deduced from cDNA | Antarctic bacterium TAB5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Antarctic bacterium TAB5 | Q9KWY4 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Q-Sepharose fast flow column and a Sephacryl S200 high-resolution gel-filtration column | Antarctic bacterium TAB5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Antarctic bacterium TAB5 | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Antarctic bacterium TAB5 |
TAB5 AP | - |
Antarctic bacterium TAB5 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Antarctic bacterium TAB5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
residual activity after after thermal inactivation at 60°C, 120 min: 2.4% | Antarctic bacterium TAB5 |
10 | 30 | - |
Antarctic bacterium TAB5 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S86A/G87A | Antarctic bacterium TAB5 | |
70 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S42G/H135E | Antarctic bacterium TAB5 | |
71 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S42G/S338T | Antarctic bacterium TAB5 | |
100 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant G87A | Antarctic bacterium TAB5 | |
114 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S338T | Antarctic bacterium TAB5 | |
117 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant G149D | Antarctic bacterium TAB5 | |
133 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S42G | Antarctic bacterium TAB5 | |
203 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant S86A | Antarctic bacterium TAB5 | |
236 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, wild-type | Antarctic bacterium TAB5 | |
303 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant H135E | Antarctic bacterium TAB5 | |
361 | - |
4-nitrophenyl phosphate | pH 10.0, 15°C, mutant H135E/G149D | Antarctic bacterium TAB5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Antarctic bacterium TAB5 |