Literature summary for 3.1.3.1 extracted from

Orimo, H.; Goseki-Sone, M.; Hosoi, T.; Shimada, T.
Functional assay of the mutant tissue-nonspecific alkaline phosphatase gene using U2OS osteoblast-like cells (2008), Mol. Genet. Metab., 94, 375-381.

Search for more literature for 3.1.3.1

Protein Variants

Protein Variants	Comment	Organism
A115V	activity in U2OS cells after 48h after transfection: 0.1%	Homo sapiens
additional information	to determine the mineralizing ability of mutant TNAP proteins, a functional assay is developed that uses U2OS osteoblast-like cells. Expression plasmids containing TNAP mutant cDNAs are constructed and introduced into U2OS cells, which are derived from a human osteosarcoma and exhibit very low alkaline phosphatase (ALP) activity and disabled mineralization. U2OS cells, in which active TNAP cDNAs are introduced, express high ALP activity and mineralized their circumstance when they are cultured with beta-glycerophosphate. An in vitro mineralization assay of U2OS cells transfected with moderate allele cDNAs shows that approximately 35% of TNAP enzymatic activity may be the threshold value for mineralization. In addition, U2OS cells transfected with wild-type TNAP and polymorphism TNAP cDNA shows PHEX (phosphate-regulating gene with homologies to endopeptidases on the X chromosome) induction as in SaOS-2 cells	Homo sapiens
R54A	activity in U2OS cells after 48h after transfection: 0.1%	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Homo sapiens	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
tissue-nonspecific alkaline phosphatase	-	Homo sapiens
TNAP	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)