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Literature summary for 3.1.3.1 extracted from
- Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum (2008), J. Plant Physiol., 165, 1655-1666.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | relationship between extracellular pH and the release of alkaline phosphatase using stimuli affecting extracellular pH, i.e. heat shock, nigericin, salicylicacid, benzoic acid, fusicoccin, NaOH, KOH, HCl, and the Golgi apparatus inhibitor BrefeldinA is investigated. Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation | Oxybasis rubra |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| molybdate | 10 mM, activity of heat-shocked induced phosphatase: -33% | Oxybasis rubra |  |
| phosphate | 2.5 mM, activity of heat-shocked induced phosphatase: -64% | Oxybasis rubra | |
| ZnCl2 | 0.01 mM, activity of heat-shocked induced phosphatase: -82% | Oxybasis rubra | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Oxybasis rubra | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oxybasis rubra | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
extracellular alkaline phosphatase shows 3fold more activity with tyrosine phosphate in comparison to p-nitrophenylphosphate | Oxybasis rubra |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Oxybasis rubra | 4-nitrophenol + phosphate | - |
? | |
| tyrosine phosphate + H2O | - |
Oxybasis rubra | tyrosine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular alkaline phosphatase | - |
Oxybasis rubra |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
pH-optimum of heat-shock-induced phosphatase | Oxybasis rubra |
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