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Literature summary for 3.1.3.1 extracted from
- Purification and biochemical characterization of thermostable alkaline phosphatases produced by Rhizopus microsporus var. rhizopodiformis (2008), Folia Microbiol. (Praha), 53, 509-516.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
4-nitrophenyl phosphate | intracellular form | Rhizopus microsporus |  |
| 0.28 | - |
4-nitrophenyl phosphate | intracellular form | Rhizopus microsporus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Rhizopus microsporus | - |
- |
| intracellular | - |
Rhizopus microsporus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | activates only the extracellular form | Rhizopus microsporus | |
| Mg2+ | stimulates activity of extra- and intracellular form | Rhizopus microsporus | |
| Mn2+ | stimulates activity of extra- and intracellular form | Rhizopus microsporus | |
| Na+ | stimulates activity of extra- and intracellular form | Rhizopus microsporus | |
| Zn2+ | stimulates activity of extra- and intracellular form | Rhizopus microsporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Rhizopus microsporus |
| 118000 | - |
gel filtration, intracellular | Rhizopus microsporus |
| 120000 | - |
gel filtration, extracellular | Rhizopus microsporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus microsporus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using DEAE-cellulose and ConA-sepharose chromatography | Rhizopus microsporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Rhizopus microsporus | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Rhizopus microsporus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AIP | - |
Rhizopus microsporus |
| alkaline phosphatase | - |
Rhizopus microsporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Rhizopus microsporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhizopus microsporus |
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