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Literature summary for 3.1.3.1 extracted from

  • Orhanovic, S.; Bucevic-Popovic, V.; Pavela-Vrancic, M.; Vujaklija, D.; Gamulin, V.
    Effect of a T81A mutation at the subunit interface on catalytic properties of alkaline phosphatase from Escherichia coli (2006), Int. J. Biol. Macromol., 40, 54-58.
    View publication on PubMed
Search for more literature for 3.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
T81A the T81A mutant displays a somewhat lower affinity than the wild-type enzyme for both substrate and phosphate, while kcat does not change significantly Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 4-nitrophenyl phosphate 25°C, pH 8, mutant enzyme T81A Escherichia coli
0.0078
-
 4-nitrophenyl phosphate 25°C, pH 8, wild-type enzyme Escherichia coli
0.16
-
 4-nitrophenyl phosphate 25°C, pH 8, wild-type enzyme Escherichia coli
0.23
-
 4-nitrophenyl phosphate 25°C, pH 8, mutant enzyme T81A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 4-nitrophenyl phosphate 25°C, pH 8, mutant enzyme T81A Escherichia coli
18
-
 4-nitrophenyl phosphate 25°C, pH 10.5, mutant enzyme T81A Escherichia coli
24.39
-
 4-nitrophenyl phosphate 25°C, pH 10.5, mutant enzyme T81A Escherichia coli
26
-
 4-nitrophenyl phosphate 25°C, pH10.5, wild-type enzyme Escherichia coli
34
-
 4-nitrophenyl phosphate 25°C, pH 8, wild-type enzyme Escherichia coli
36.98
-
 4-nitrophenyl phosphate 25°C, pH 8, mutant enzyme T81A Escherichia coli
61.84
-
 4-nitrophenyl phosphate 25°C, pH 8, wild-type enzyme Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0061
-
 phosphate 25°C, pH 8, wild-type enzyme Escherichia coli
0.0065
-
 phosphate 25°C, pH 8, mutant enzyme T81A Escherichia coli
0.1
-
 phosphate 25°C, pH 10.5, wild-type enzyme Escherichia coli
0.14
-
 phosphate 25°C, pH 10.5, mutant enzyme T81A Escherichia coli