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Literature summary for 3.1.3.1 extracted from

  • Asgeirsson, B.; Adalbjoernsson, B.V.; Gylfason, G.A.
    Engineered disulfide bonds increase active-site local stability and reduce catalytic activity of a cold-adapted alkaline phosphatase (2007), Biochim. Biophys. Acta, 1774, 679-687.
    View publication on PubMed
Search for more literature for 3.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.

Protein Variants

Protein Variants Comment Organism
G51C kcat for 4-nitrophenyl phosphate is 15.8fold lower than wild-type enzyme. Km-value for 4-nitrophenyl phosphate is 2.8fold lower than wild-type value Vibrio sp.
I50C kcat for 4-nitrophenyl phosphate is 6fold lower than wild-type enzyme. Km-value for 4-nitrophenyl phosphate is 2.4fold lower than wild-type value Vibrio sp.
S52C kcat for 4-nitrophenyl phosphate is 10.2fold lower than wild-type enzyme. Km-value for 4-nitrophenyl phosphate is 1.9fold lower than wild-type value Vibrio sp.
S53C kcat for 4-nitrophenyl phosphate is 9.6fold lower than wild-type enzyme. Km-value for 4-nitrophenyl phosphate is 1.9fold lower than wild-type value Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
phosphate competitive Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme G51C Vibrio sp.
0.046
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme I50C Vibrio sp.
0.058
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme S52C Vibrio sp.
0.059
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme S53C Vibrio sp.
0.11
-
 4-nitrophenyl phosphate 25°C, pH 8.0, wild-.type enzyme Vibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Vibrio sp.
-
-

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Vibrio sp. 4-nitrophenol + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 30 min, 50% loss of wild-type activity at 19.5°C Vibrio sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
132
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme G51C Vibrio sp.
204
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme S52C Vibrio sp.
217
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme S53C Vibrio sp.
350
-
 4-nitrophenyl phosphate 25°C, pH 8.0, mutant enzyme I50C Vibrio sp.
2086
-
 4-nitrophenyl phosphate 25°C, pH 8.0, wild-type enzyme Vibrio sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.027
-
 phosphate 25°C, pH 8.0, mutant enzyme G51C Vibrio sp.
0.03
-
 phosphate 25°C, pH 8.0, mutant enzyme I50C Vibrio sp.
0.034
-
 phosphate 25°C, pH 8.0, mutant enzyme S52C Vibrio sp.
0.1
-
 phosphate 25°C, pH 8.0, mutant enzyme S53C Vibrio sp.
0.1
-
 phosphate 25°C, pH 8.0, wild-type enzyme Vibrio sp.