Application | Comment | Organism |
---|---|---|
analysis | development of a simple in situ duodenal alkaline phosphatase activity assay based on the fluorogenic substrate ELF | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Cys | 10 mM | Rattus norvegicus | |
glycerol phosphate | - |
Rattus norvegicus | |
L-Cys | - |
Rattus norvegicus | |
L-Phe | maximal ainhibition at 1 mM. D-Phe has no effect | Rattus norvegicus | |
levamisole | inhibits brush border membrane enzyme activity in the upper villus but is less effective in the middle villus and has no effect on alkaline phosphatase activity in the mucus gel | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | villous surface. HCO3- secretion increases alkaline phosphatase activity by increasing local pH at its catalytic site. Alkaline phosphatase hydrolyzes luminal phosphates, presumably ATP, which increases HCO3- secretion via activation of P2 receptors | Rattus norvegicus | - |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Rattus norvegicus |