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Literature summary for 3.1.3.1 extracted from
- Expression and characterization of recombinant thermostable alkaline phosphatase from a novel thermophilic bacterium Thermus thermophilus XM (2007), Acta Biochim. Biophys. Sin., 39, 844-850.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His6-tagged fusion protein in Escherichia coli | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, 16% inhibition | Thermus thermophilus |  |
| Co2+ | 2 mM, 25% inhibition | Thermus thermophilus | |
| Cu2+ | 2 mM, 29% inhibition | Thermus thermophilus | |
| Fe2+ | 2 mM, 89% inhibition | Thermus thermophilus | |
| Mn2+ | 2 mM, 32% inhibition | Thermus thermophilus | |
| Zn2+ | 2 mM, 51% inhibition | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
4-nitrophenyl phosphate | 75°C | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 0.1 mM, 2.4fold increase in activity | Thermus thermophilus | |
| Fe2+ | 0.1 mM, 1.25fold increase in activity | Thermus thermophilus | |
| Mg2+ | 0.1 mM, 1.3fold increase in activity. 2 mM, 1.6fold increase in activity | Thermus thermophilus | |
| Mn2+ | 0.1 mM, 1.3fold increase in activity | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54700 | - |
x * 54700, calculated from sequence | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q153J0 | - |
- |
| Thermus thermophilus XM | Q153J0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus XM | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54700, calculated from sequence | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | - |
Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 90 | 45°C: about 50% of maximal activity, 90°C: about 70% of maximal activity | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
270 min, about 10% loss of activity | Thermus thermophilus |
| 80 | - |
retains more than 50% activity after incubating for 6 h at 80ºC | Thermus thermophilus |
| 95 | - |
60 min, about 45% loss of activity | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 12 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 11 | 12.5 | 11.0: about 50% of maximal activity, pH 12.5: about 65% of maximal activity | Thermus thermophilus |
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