Application | Comment | Organism |
---|---|---|
biotechnology | the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasinf their activity | Pandalus borealis |
biotechnology | the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasing their activity | Bos taurus |
General Stability | Organism |
---|---|
improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives | Bos taurus |
improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives | Pandalus borealis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-nitrophenyl phosphate | 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction | Pandalus borealis | |
0.11 | - |
4-nitrophenyl phosphate | 37°C, native enzyme | Bos taurus | |
0.12 | - |
4-nitrophenyl phosphate | 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction | Pandalus borealis | |
0.13 | - |
4-nitrophenyl phosphate | 37°C, native enzyme | Pandalus borealis | |
0.17 | - |
4-nitrophenyl phosphate | 57°C, native enzyme | Bos taurus | |
0.18 | - |
4-nitrophenyl phosphate | 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
0.2 | - |
4-nitrophenyl phosphate | 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
0.23 | - |
4-nitrophenyl phosphate | 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
0.26 | - |
4-nitrophenyl phosphate | 10°C, native enzyme | Pandalus borealis | |
0.85 | - |
4-nitrophenyl phosphate | 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Pandalus borealis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Pandalus borealis | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAP | - |
Bos taurus |
SAP | - |
Pandalus borealis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
native enzyme and enzymes with chemically modified aliphatic or amino groups using tetracarboxy-benzophenone derivatives | Pandalus borealis |
56 | - |
native enzyme and enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
58 | - |
enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
t1/2: 23 min, native enzyme | Pandalus borealis |
50 | - |
t1/2: 182 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) | Pandalus borealis |
50 | - |
t1/2: 38 min, native enzyme | Bos taurus |
50 | - |
t1/2: 58 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
50 | - |
t1/2: 77 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
53 | - |
t1/2: 15 min, native enzyme | Bos taurus |
53 | - |
t1/2: 20 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
53 | - |
t1/2: 26 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
55 | - |
t1/2: 2.75 min, native enzyme | Pandalus borealis |
55 | - |
t1/2: 41 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) | Pandalus borealis |
56 | - |
t1/2: 10 min, native enzyme | Bos taurus |
56 | - |
t1/2: 12 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
56 | - |
t1/2: 14 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
58 | - |
t1/2: 1 min, native enzyme | Pandalus borealis |
58 | - |
t1/2: 80 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
58 | - |
t1/2: 84 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
59 | - |
t1/2: 6 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
59 | - |
t1/2: 6 min, native enzyme | Bos taurus |
59 | - |
t1/2: 7 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |
60 | - |
t1/2: 0.7 min, native enzyme | Pandalus borealis |
60 | - |
t1/2: 10 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) | Pandalus borealis |
62 | - |
t1/2: 35 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
62 | - |
t1/2: 37 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
65 | - |
t1/2: 1 min, native enzyme | Bos taurus |
65 | - |
t1/2: 2.6 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) | Pandalus borealis |
65 | - |
t1/2: 24 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
65 | - |
t1/2: 25 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
69 | - |
t1/2: 6 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
73 | - |
t1/2: 4 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | 0.23 | 4-nitrophenyl phosphate | 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
0.55 | - |
4-nitrophenyl phosphate | 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction | Pandalus borealis | |
14.67 | - |
4-nitrophenyl phosphate | 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction | Pandalus borealis | |
20 | 50 | 4-nitrophenyl phosphate | 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
65 | - |
4-nitrophenyl phosphate | 37°C, native enzyme | Bos taurus | |
77.4 | - |
4-nitrophenyl phosphate | 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
78.83 | - |
4-nitrophenyl phosphate | 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
87.83 | - |
4-nitrophenyl phosphate | 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Bos taurus | |
115.9 | - |
4-nitrophenyl phosphate | 37°C, native enzyme | Bos taurus | |
153.9 | - |
4-nitrophenyl phosphate | 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction | Pandalus borealis | |
155.7 | - |
4-nitrophenyl phosphate | 10°C, native enzyme | Pandalus borealis | |
183.3 | - |
4-nitrophenyl phosphate | 57°C, native enzyme | Bos taurus | |
812.3 | - |
4-nitrophenyl phosphate | 37°C, native enzyme | Pandalus borealis | |
1443 | - |
4-nitrophenyl phosphate | 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis | |
16230 | - |
4-nitrophenyl phosphate | 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride | Pandalus borealis |