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Literature summary for 3.1.3.1 extracted from

  • Siddiqui, K.S.; Poljak, A.; Cavicchioli, R.
    Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives (2004), Cell. Mol. Biol., 50, 657-667.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasinf their activity Pandalus borealis
biotechnology the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasing their activity Bos taurus

General Stability

General Stability Organism
improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives Bos taurus
improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives Pandalus borealis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
0.11
-
4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
0.12
-
4-nitrophenyl phosphate 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
0.13
-
4-nitrophenyl phosphate 37°C, native enzyme Pandalus borealis
0.17
-
4-nitrophenyl phosphate 57°C, native enzyme Bos taurus
0.18
-
4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
0.2
-
4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
0.23
-
4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
0.26
-
4-nitrophenyl phosphate 10°C, native enzyme Pandalus borealis
0.85
-
4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Pandalus borealis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Pandalus borealis 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
CAP
-
Bos taurus
SAP
-
Pandalus borealis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
native enzyme and enzymes with chemically modified aliphatic or amino groups using tetracarboxy-benzophenone derivatives Pandalus borealis
56
-
native enzyme and enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
58
-
enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
t1/2: 23 min, native enzyme Pandalus borealis
50
-
t1/2: 182 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
50
-
t1/2: 38 min, native enzyme Bos taurus
50
-
t1/2: 58 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
50
-
t1/2: 77 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
53
-
t1/2: 15 min, native enzyme Bos taurus
53
-
t1/2: 20 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
53
-
t1/2: 26 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
55
-
t1/2: 2.75 min, native enzyme Pandalus borealis
55
-
t1/2: 41 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
56
-
t1/2: 10 min, native enzyme Bos taurus
56
-
t1/2: 12 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
56
-
t1/2: 14 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
58
-
t1/2: 1 min, native enzyme Pandalus borealis
58
-
t1/2: 80 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
58
-
t1/2: 84 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
59
-
t1/2: 6 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
59
-
t1/2: 6 min, native enzyme Bos taurus
59
-
t1/2: 7 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
60
-
t1/2: 0.7 min, native enzyme Pandalus borealis
60
-
t1/2: 10 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
62
-
t1/2: 35 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
62
-
t1/2: 37 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
65
-
t1/2: 1 min, native enzyme Bos taurus
65
-
t1/2: 2.6 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
65
-
t1/2: 24 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
65
-
t1/2: 25 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
69
-
t1/2: 6 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
73
-
t1/2: 4 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037 0.23 4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
0.55
-
4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
14.67
-
4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
20 50 4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
65
-
4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
77.4
-
4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
78.83
-
4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
87.83
-
4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
115.9
-
4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
153.9
-
4-nitrophenyl phosphate 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
155.7
-
4-nitrophenyl phosphate 10°C, native enzyme Pandalus borealis
183.3
-
4-nitrophenyl phosphate 57°C, native enzyme Bos taurus
812.3
-
4-nitrophenyl phosphate 37°C, native enzyme Pandalus borealis
1443
-
4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
16230
-
4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis