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Literature summary for 3.1.3.1 extracted from

  • Terefe, N.S.; Arimi, J.M.; Van Loey, A.; Hendrickx, M.
    Kinetics of the alkaline phosphatase catalyzed hydrolysis of disodium p-nitrophenyl phosphate: effects of carbohydrate additives, low temperature, and freezing (2004), Biotechnol. Prog., 20, 1467-1478.
    View publication on PubMed

General Stability

General Stability Organism
carbohydrate additives such as sucrose may result in a remarkable higher reduction in the kinetics of deteriorative reactions and result in a better stability Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.48
-
4-nitrophenyl phosphate 40% sucrose, -4.5°C, frozen Bos taurus
2.97
-
4-nitrophenyl phosphate 40% sucrose, -4.5°C, unfrozen Bos taurus
6.4
-
4-nitrophenyl phosphate 40% sucrose, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O reaction is at least partially diffusion-controlled Bos taurus 4-nitrophenol + phosphate
-
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