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Literature summary for 3.1.3.1 extracted from

  • Ishibashi, M.; Yamashita, S.; Tokunaga, M.
    Characterization of halophilic alkaline phosphatase from Halomonas sp. 593, a moderately halophilic bacterium (2005), Biosci. Biotechnol. Biochem., 69, 1213-1216.
    View publication on PubMed
Search for more literature for 3.1.3.1

General Stability

General Stability Organism
enzyme is partially resistant to SDS, without heat treatment 40% of the enzyme is SDS-resistant Halomonas sp.
requires at least 0.3 M NaCl for stability Halomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 70% inhibition Halomonas sp.
NaCl 2.0 M, 71% loss of activity Halomonas sp.
Zn2+ 2 mM, 90% inhibition Halomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM, 2.1fold stimulation Halomonas sp.
Mn2+ 2 mM, 1.7fold stimulation Halomonas sp.
additional information optimal activity in absence of NaCl Halomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 gel filtration Halomonas sp.

Organism

Organism UniProt Comment Textmining
Halomonas sp.
-
-
-

Subunits

Subunits Comment Organism
dimer
-
 Halomonas sp.

Synonyms

Synonyms Comment Organism
HaALP
-
 Halomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 50
-
 Halomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, about 80% loss of activity Halomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Halomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 stable Halomonas sp.