KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, wild-type enzyme | Thermus yunnanensis | |
1.43 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, Co2+-combined enzyme | Thermus yunnanensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity is dependent on metal ion, Co2+ is the most active stimulator and has unique effect at high temperature | Thermus yunnanensis | |
Mg2+ | activity is dependent on metal ion, Co2+, Mg2+ and Zn2+ are the main activators | Thermus yunnanensis | |
Zn2+ | activity is dependent on metal ion, Co2+, Mg2+ and Zn2+ are the main activators | Thermus yunnanensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Thermus yunnanensis |
104000 | - |
- |
Thermus yunnanensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus yunnanensis | - |
sp. nov. | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus yunnanensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3200 | - |
- |
Thermus yunnanensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Thermus yunnanensis | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Thermus yunnanensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermus yunnanensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 95 | 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity | Thermus yunnanensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life: 803 min | Thermus yunnanensis |
70 | - |
half-life: 527 min | Thermus yunnanensis |
80 | - |
half-life: 199 min, Mg2+ can increase half-life of the enzyme twice | Thermus yunnanensis |
90 | - |
half-life: 18 min | Thermus yunnanensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, wild-type enzyme | Thermus yunnanensis | |
1130 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, Co2+-combined enzyme | Thermus yunnanensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thermus yunnanensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | pH 8.5: about 50% of maximal activity, about 55% of maximal activity | Thermus yunnanensis |