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Literature summary for 3.1.3.1 extracted from

  • Galabova, D.; Tuleva, B.; Vasileva-Tonkova, E.; Christova, N.
    Purification and properties of alkaline phosphatase with protein phosphatase activity from Saccharomyces cerevisiae (2000), Z. Naturforsch. C, 55, 588-593.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 slight activation at 10 mM Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Saccharomyces cerevisiae
Ca2+
-
Saccharomyces cerevisiae
EDTA
-
Saccharomyces cerevisiae
L-cysteine
-
Saccharomyces cerevisiae
Mn2+
-
Saccharomyces cerevisiae
o-phenanthroline
-
Saccharomyces cerevisiae
phosphate conpetitive Saccharomyces cerevisiae
Tartrate weak inhibition at 10 mM Saccharomyces cerevisiae
Zn2+ inhibition at 10 mM, activation at 1 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
4-nitrophenyl phosphate
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2fold activation at 10 mM, dependent on, if 4-nitrophenyl phosphate is used as a substrate, protein phosphatase activity is not Mg2+-dependent Saccharomyces cerevisiae
additional information not affected by F- at 10 mM, protein phosphatase activity is not Mg2+-dependent Saccharomyces cerevisiae
Na+ slight activation at 10 mM Saccharomyces cerevisiae
Zn2+ inhibition at 10 mM, activation at 1 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000, SDS-PAGE Saccharomyces cerevisiae
130000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae 257
-
-
-

Purification (Commentary)

Purification (Comment) Organism
345fold Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54.53
-
purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
Saccharomyces cerevisiae adenosine + phosphate
-
?
3'-AMP + H2O
-
Saccharomyces cerevisiae 257 adenosine + phosphate
-
?
4-nitrophenyl phosphate + H2O best substrate Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
?
5'-AMP + H2O
-
Saccharomyces cerevisiae adenosine + phosphate
-
?
5'-AMP + H2O
-
Saccharomyces cerevisiae 257 adenosine + phosphate
-
?
ADP + H2O
-
Saccharomyces cerevisiae AMP + phosphate
-
?
alpha-glycerophosphate + H2O low activity with alpha-isomer Saccharomyces cerevisiae glycerol + phosphate
-
?
alpha-naphthyl phosphate + H2O
-
Saccharomyces cerevisiae alpha-naphthol + phosphate
-
?
ATP + H2O
-
Saccharomyces cerevisiae ADP + phosphate
-
?
beta-glycerophosphate + H2O
-
Saccharomyces cerevisiae glycerol + phosphate
-
?
beta-glycerophosphate + H2O
-
Saccharomyces cerevisiae 257 glycerol + phosphate
-
?
casein + H2O enzyme shows protein phosphatase activity Saccharomyces cerevisiae ?
-
?
casein + H2O enzyme shows protein phosphatase activity Saccharomyces cerevisiae 257 ?
-
?
D-glucose 1-phosphate + H2O
-
Saccharomyces cerevisiae D-glucose + phosphate
-
?
D-glucose 6-phosphate + H2O
-
Saccharomyces cerevisiae D-glucose + phosphate
-
?
histone II-A + H2O enzyme shows protein phosphatase activity Saccharomyces cerevisiae ?
-
?
additional information non-specific enzyme, wide substrate specificity, no phosphate transfer with alcohols, except for Tris Saccharomyces cerevisiae ?
-
?
additional information non-specific enzyme, wide substrate specificity, no phosphate transfer with alcohols, except for Tris Saccharomyces cerevisiae 257 ?
-
?
O-phospho-DL-serine + H2O
-
Saccharomyces cerevisiae DL-serine + phosphate
-
?
O-phospho-DL-tyrosine + H2O
-
Saccharomyces cerevisiae DL-tyrosine + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Saccharomyces cerevisiae
ALPase
-
Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable at Saccharomyces cerevisiae
70
-
1% remaining activity after 10 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 8.8 at 37°C Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
phosphate
-
Saccharomyces cerevisiae