Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | slight activation at 10 mM | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Saccharomyces cerevisiae | |
Ca2+ | - |
Saccharomyces cerevisiae | |
EDTA | - |
Saccharomyces cerevisiae | |
L-cysteine | - |
Saccharomyces cerevisiae | |
Mn2+ | - |
Saccharomyces cerevisiae | |
o-phenanthroline | - |
Saccharomyces cerevisiae | |
phosphate | conpetitive | Saccharomyces cerevisiae | |
Tartrate | weak inhibition at 10 mM | Saccharomyces cerevisiae | |
Zn2+ | inhibition at 10 mM, activation at 1 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-nitrophenyl phosphate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2fold activation at 10 mM, dependent on, if 4-nitrophenyl phosphate is used as a substrate, protein phosphatase activity is not Mg2+-dependent | Saccharomyces cerevisiae | |
additional information | not affected by F- at 10 mM, protein phosphatase activity is not Mg2+-dependent | Saccharomyces cerevisiae | |
Na+ | slight activation at 10 mM | Saccharomyces cerevisiae | |
Zn2+ | inhibition at 10 mM, activation at 1 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
130000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae 257 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
345fold | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.53 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | - |
Saccharomyces cerevisiae | adenosine + phosphate | - |
? | |
3'-AMP + H2O | - |
Saccharomyces cerevisiae 257 | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | best substrate | Saccharomyces cerevisiae | 4-nitrophenol + phosphate | - |
? | |
5'-AMP + H2O | - |
Saccharomyces cerevisiae | adenosine + phosphate | - |
? | |
5'-AMP + H2O | - |
Saccharomyces cerevisiae 257 | adenosine + phosphate | - |
? | |
ADP + H2O | - |
Saccharomyces cerevisiae | AMP + phosphate | - |
? | |
alpha-glycerophosphate + H2O | low activity with alpha-isomer | Saccharomyces cerevisiae | glycerol + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | - |
Saccharomyces cerevisiae | alpha-naphthol + phosphate | - |
? | |
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
beta-glycerophosphate + H2O | - |
Saccharomyces cerevisiae | glycerol + phosphate | - |
? | |
beta-glycerophosphate + H2O | - |
Saccharomyces cerevisiae 257 | glycerol + phosphate | - |
? | |
casein + H2O | enzyme shows protein phosphatase activity | Saccharomyces cerevisiae | ? | - |
? | |
casein + H2O | enzyme shows protein phosphatase activity | Saccharomyces cerevisiae 257 | ? | - |
? | |
D-glucose 1-phosphate + H2O | - |
Saccharomyces cerevisiae | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Saccharomyces cerevisiae | D-glucose + phosphate | - |
? | |
histone II-A + H2O | enzyme shows protein phosphatase activity | Saccharomyces cerevisiae | ? | - |
? | |
additional information | non-specific enzyme, wide substrate specificity, no phosphate transfer with alcohols, except for Tris | Saccharomyces cerevisiae | ? | - |
? | |
additional information | non-specific enzyme, wide substrate specificity, no phosphate transfer with alcohols, except for Tris | Saccharomyces cerevisiae 257 | ? | - |
? | |
O-phospho-DL-serine + H2O | - |
Saccharomyces cerevisiae | DL-serine + phosphate | - |
? | |
O-phospho-DL-tyrosine + H2O | - |
Saccharomyces cerevisiae | DL-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Saccharomyces cerevisiae |
ALPase | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable at | Saccharomyces cerevisiae |
70 | - |
1% remaining activity after 10 min | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 8.8 | at 37°C | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
phosphate | - |
Saccharomyces cerevisiae |