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Literature summary for 3.1.3.1 extracted from
- Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity (2002), Protein Sci., 11, 903-911.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 90% inhibition at 1 mM and 25°C, complete inhibition at 0.1 mM EDTA and 90°C | Thermotoga maritima |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
4-nitrophenyl phosphate | pH 8.0, 65°C | Thermotoga maritima | |
| 0.175 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | metalloenzyme, dependent on, can partially be substituted by Mg2+ and Mn2+ | Thermotoga maritima | |
| Mg2+ | metalloenzyme, can partially substitute for Co2+ | Thermotoga maritima | |
| Mn2+ | metalloenzyme, can partially substitute for Co2+ | Thermotoga maritima | |
| additional information | effect of metal ions, overview | Thermotoga maritima | |
| Zn2+ | metalloenzyme, 0.3 mol zinc per mol of monomer, binding structure | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli | Thermotoga maritima |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, substrate binding and active site structure | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Thermotoga maritima | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Thermotoga maritima |
| AP | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 90 | - |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for more than 24 h | Thermotoga maritima |
| 90 | - |
stable for 5 h | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C | Thermotoga maritima | |
| 100 | - |
4-nitrophenyl phosphate | pH 8.0, 65°C | Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermotoga maritima |
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Release 2023.1 (January 2023)
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