Cloned (Comment) | Organism |
---|---|
94% fragment of gene alp cDNA, functional overexpression in Escherichia coli BL21(DE3) as internally His-tagged enzyme use of continous electrophoresis procedure | Dictyostelium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, recombinant enzyme, SDS-PAGE | Dictyostelium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium sp. | - |
gene alp | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme in nclusion bodies after expression in Escherichia coli | Dictyostelium sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Dictyostelium sp. |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from inclusion bodies after expression in Escherichia coli, solubilization by 1% SDS in ddH2O from purified inclusion bodies | Dictyostelium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
purified enzyme, substrate cAMP | Dictyostelium sp. |
0.33 | - |
purified enzyme, substrate ATP | Dictyostelium sp. |
0.65 | - |
purified enzyme, substrate beta-glycerol phosphate | Dictyostelium sp. |
0.7 | - |
purified enzyme, substrates 4-nitrophenyl phosphate and 5'-AMP | Dictyostelium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Dictyostelium sp. | 4-nitrophenol + phosphate | - |
? | |
5'-AMP + H2O | - |
Dictyostelium sp. | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Dictyostelium sp. | ADP + phosphate | - |
? | |
beta-glycerol phosphate + H2O | - |
Dictyostelium sp. | glycerol + phosphate | - |
? | |
cAMP + H2O | - |
Dictyostelium sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, recombinant enzyme, SDS-PAGE | Dictyostelium sp. |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Dictyostelium sp. |
ALP | - |
Dictyostelium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dictyostelium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
approximately | Dictyostelium sp. |