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Literature summary for 3.1.3.1 extracted from
- Heat-labile bacterial alkaline phosphatase from a marine Vibrio sp (2000), Enzyme Microb. Technol., 27, 66-73.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inactivation after 1 h at 1 mM | Vibrio sp. |  |
| phosphate | competitive | Vibrio sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics at several temperatures and pH-value combinations | Vibrio sp. | |
| 0.96 | - |
4-nitrophenyl phosphate | pH 10.0, 25°C | Vibrio sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion to the culture medium | Vibrio sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metalloenzyme | Vibrio sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | 55000 | gel filtration and native PAGE | Vibrio sp. |
| 55000 | - |
1 * 55000, SDS-PAGE | Vibrio sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio sp. | - |
Vibrio 2P44 | - |
| Vibrio sp. 2P44 | - |
Vibrio 2P44 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from strain G15-21, 151fold from the culture medium | Vibrio sp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Vibrio sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3707 | - |
purified enzyme | Vibrio sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Vibrio sp. | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Vibrio sp. 2P44 | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Vibrio sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Vibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Vibrio sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
fully stable below | Vibrio sp. |
| 32 | - |
half-life 30 min | Vibrio sp. |
| 40 | - |
half-life 6 min | Vibrio sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Vibrio sp. | |
| 350 | - |
4-nitrophenyl phosphate | pH 10.0, 25°C | Vibrio sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Vibrio sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
phosphate | pH 8.0, 25°C | Vibrio sp. | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vibrio sp. | isoelectric focusing, strain G15-21 | - |
7.6 |
| Vibrio sp. | isoelectric focusing in presence of 8 M urea and 1.25 mM DTT, strain G15-21 | - |
9.5 |
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