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Literature summary for 3.1.3.1 extracted from
- Acid and alkaline phosphatase activities in the clam Scrobicularia plana: kinetic characteristics and effects of heavy metals (2002), Comp. Biochem. Physiol. B, 131, 241-249.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 39% inhibition of the enzyme at 1 mM in the gills, but not in digestive gland | Scrobicularia plana |  |
| Cu2+ | - |
Scrobicularia plana | |
| Hg2+ | - |
Scrobicularia plana | |
| additional information | inhibition level is tissue-dependent | Scrobicularia plana | |
| Zn2+ | about 30% inhibition at 1 mM in gill, mantle, and siphon | Scrobicularia plana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Scrobicularia plana | |
| 1.51 | - |
4-nitrophenyl phosphate | pH 9.5, 30°C, foot | Scrobicularia plana | |
| 2.48 | - |
4-nitrophenyl phosphate | pH 8.5, 30°C, digestive gland and siphon | Scrobicularia plana | |
| 2.88 | - |
4-nitrophenyl phosphate | pH 8.5, 30°C, mantle | Scrobicularia plana | |
| 4.52 | - |
4-nitrophenyl phosphate | pH 8.5, 30°C, gills | Scrobicularia plana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scrobicularia plana | - |
clam, Da Costa 1778 | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Scrobicularia plana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | highest enzyme activity | Scrobicularia plana | - |
| foot | - |
Scrobicularia plana | - |
| gill | - |
Scrobicularia plana | - |
| mantle | - |
Scrobicularia plana | - |
| siphon | - |
Scrobicularia plana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Scrobicularia plana | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Scrobicularia plana |
| ALP | - |
Scrobicularia plana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Scrobicularia plana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Scrobicularia plana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, nearly complete loss of activity | Scrobicularia plana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Scrobicularia plana |
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