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Literature summary for 3.1.26.8 extracted from

  • Pace, B.; Stahl, D.A.; Pace, N.R.
    The catalytic element of a ribosomal RNA-processing complex (1984), J. Biol. Chem., 259, 11454-11458.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00003
-
RNA in complex with BL16 subunit
-
Bacillus subtilis
0.00015
-
RNA
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5S rRNA precursor + H2O the enzyme attacks a ribonucleoprotein substrate consisting of protein BL16 in complex with the 5S rRNA precursor Bacillus subtilis mature 5S rRNA + oligonucleotides
-
?
artificial 5S rRNA precursor + H2O
-
Bacillus subtilis ?
-
?
artificial 5S rRNA precursor substrate in complex with BL16 subunit + H2O
-
Bacillus subtilis ?
-
?
RNA + H2O
-
Bacillus subtilis ?
-
?
RNA in complex with BL16 subunit + H2O
-
Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
More RNase M5 complex includes two proteins. One of these proteins is ribosomal protein BL16, the other, the alpha component is required for catalysis Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00667
-
artificial 5S rRNA precursor
-
Bacillus subtilis
0.0567
-
artificial 5S rRNA precursor substrate in complex with BL16 subunit
-
Bacillus subtilis