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Literature summary for 3.1.26.4 extracted from

  • Chon, H.; Sparks, J.L.; Rychlik, M.; Nowotny, M.; Burgers, P.M.; Crouch, R.J.; Cerritelli, S.M.
    RNase H2 roles in genome integrity revealed by unlinking its activities (2013), Nucleic Acids Res., 41, 3130-3143.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D39A inactive Saccharomyces cerevisiae
G42S the mutant shows strongly reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
P45D/Y219A the mutant shows strongly reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
P45E/Y219A inactive Saccharomyces cerevisiae
P45S/Y219A inactive Saccharomyces cerevisiae
Y219A inactive Saccharomyces cerevisiae
Y219D inactive Saccharomyces cerevisiae
Y219E inactive Saccharomyces cerevisiae
Y219S inactive Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA hybrid + H2O Saccharomyces cerevisiae
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?
RNA-DNA hybrid + H2O Saccharomyces cerevisiae W-303
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae W-303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap heparin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 Saccharomyces cerevisiae ribonucleotide 5'-phosphomonoester + ?
-
 ?
RNA-DNA hybrid + H2O
-
 Saccharomyces cerevisiae W-303 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Synonyms

Synonyms Comment Organism
ribonuclease H2
-
 Saccharomyces cerevisiae
RNase H2
-
 Saccharomyces cerevisiae