Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Nguyen, T.N.; You, D.J.; Kanaya, E.; Koga, Y.; Kanaya, S.
    Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif (2013), FEBS Lett., 587, 1418-1423.
    View publication on PubMed
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured organism

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM Tris–HCl pH 8.5, 200 mM MgCl2-6H2O and 30% (w/v) PEG 4000, at 4°C uncultured organism

Protein Variants

Protein Variants Comment Organism
D22A inactive uncultured organism
D96A inactive uncultured organism
E23A inactive uncultured organism
N159A the mutant shows 20% and 2.6% activity in the presence of Mn2+ and Mg2+, respectively, compared to the wild type enzyme uncultured organism
N159D the mutant shows 17% and 5.0% activity in the presence of Mg2+ and Mn2+, respectively, compared to the wild type enzyme uncultured organism

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme prefers Mg2+ to Mn2+ ions for activity with maximal activity at 10 mM MgCl2 uncultured organism
Mn2+ the enzyme prefers Mg2+ to Mn2+ ions for activity with maximal activity at 0.1 mM MnCl2 uncultured organism

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 gel filtration uncultured organism
23800
-
 1 * 23800, calculated from amino acid sequence uncultured organism
24000
-
 1 * 24000, SDS-PAGE uncultured organism

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA hybrid + H2O uncultured organism
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured organism E0X765
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap SP column chromatography and HiTrap phenyl column chromatography uncultured organism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 uncultured organism ribonucleotide 5'-phosphomonoester + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE uncultured organism
monomer 1 * 23800, calculated from amino acid sequence uncultured organism

Synonyms

Synonyms Comment Organism
LC9-RNase H1
-
 uncultured organism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 uncultured organism

pH Range

pH Minimum pH Maximum Comment Organism
6 11 the enzyme exhibits approximately 80% of the maximal activity at pH 8.5 uncultured organism