Crystallization (Comment) | Organism |
---|---|
crystal structure analysis, overview | Escherichia coli |
crystal structure analysis, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the basic loop in Escherichia coli RNase H inhibits but does not abolish activity. If the basic loop in Escherichia coli RNase H is inserted into an isolated inactive HIV-1 RNase H domain, Mn2+-dependent activity is partially restored | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | two Mg2+ ions in the RNase H active site, required. A two-metal ion mechanism requires that metal ion A activates a water molecule as a nucleophile and moves towards ion B, bringing the nucleophile in close proximity to the scissile bond, while metal ion B destabilizes the substrate-enzyme interaction and lowers the energy barrier to product formation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | RNase H functions as an endonuclease that specifically cleaves the RNA moiety of RNA/DNA hybrids | ? | - |
? | |
additional information | Homo sapiens | RNase H functions as an endonuclease that specifically cleaves the RNA moiety of RNA/DNA hybrids. A two-metal ion mechanism requires that metal ion A activates a water molecule as a nucleophile and moves towards ion B, bringing the nucleophile in close proximity to the scissile bond, while metal ion B destabilizes the substrate-enzyme interaction and lowers the energy barrier to product formation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNase H functions as an endonuclease that specifically cleaves the RNA moiety of RNA/DNA hybrids | Escherichia coli | ? | - |
? | |
additional information | RNase H functions as an endonuclease that specifically cleaves the RNA moiety of RNA/DNA hybrids. A two-metal ion mechanism requires that metal ion A activates a water molecule as a nucleophile and moves towards ion B, bringing the nucleophile in close proximity to the scissile bond, while metal ion B destabilizes the substrate-enzyme interaction and lowers the energy barrier to product formation | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme shows a mixed beta-sheet with asymmetric alpha helices, and contains the C-helix, or basic loop, structure comparisons, overview | Escherichia coli |
More | the enzyme shows a mixed beta-sheet with asymmetric alpha helices, and contains the C-helix, or basic loop, structure comparisons, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H | - |
Escherichia coli |
ribonuclease H | - |
Homo sapiens |
RNase H | - |
Escherichia coli |
RNase H | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | RNase H exists as a free enzyme | Escherichia coli |