Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Figiel, M.; Chon, H.; Cerritelli, S.M.; Cybulska, M.; Crouch, R.J.; Nowotny, M.
    The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutieres syndrome defects (2011), J. Biol. Chem., 286, 10540-10550.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant deletion mutant AB14-233C, sitting drop vapor diffusion method at 18°C, from 0.1 M MgCl2, 15% PEG 3350, 0.1 M Bis-Tris, pH 5.5, and 2 mM reduced glutathione, X-ray diffraction structure determination and analysis at 3.1 A resolution, molecular replacement Homo sapiens

Protein Variants

Protein Variants Comment Organism
A177T naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
D2Y/L3P naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
D39Y naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
F230L naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
G37S naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
G83S naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
H86R naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
K143I naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
K162T naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
L138F naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
L60R naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
additional information mapping of the positions of 29 mutations found in Aicardi-Goutieres syndrome patients, possible effects of these mutations on the protein stability and function, overview. Construction of deletion mutants AB14–233C and ABDELTAPIPC, deletion mutant AB14-233C crystallizes more readily than the AB-PIPC and yields larger and regular crystals Homo sapiens
P138L naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
P151S naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
P43H naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
P76L naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R108W naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R13H naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R186W naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R235Q naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R291H naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
R69W naturally occuring mutation in subunit C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
S159I naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
S229P naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
T163I naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
T240M naturally occuring mutation in subunit A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
V183M naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
V185G naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
W73L naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens
Y219H naturally occuring mutation in subunit B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, best at 10 mM Homo sapiens
Mn2+ required, best at 1 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the eukaryotic RNase H2 heterotrimeric complex recognizes RNA/DNA hybrids and 5'RNA-DNA3'/DNA junction hybrids as substrates with similar efficiency ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the eukaryotic RNase H2 heterotrimeric complex recognizes RNA/DNA hybrids and 5'RNA-DNA3'/DNA junction hybrids as substrates with similar efficiency Homo sapiens ?
-
 ?
additional information substrate is [alpha-32P]ATP-labeled poly(rA)/poly(dT) Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H2
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 comparison of thermostability of wild-type and mutant enzymes, overview Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function RNase H2 cleaves RNA sequences that are part of RNA/DNA hybrids or that are incorporated into DNA, thus, preventing genomic instability and the accumulation of aberrant nucleic acid, which in humans induces Aicardi-Goutieres syndrome, a severe autoimmune disorder Homo sapiens