Cloned (Comment) | Organism |
---|---|
simultaneous expression of GST-tagged RNASEH2B, and untagged RNASEH2A and RNASEH2C subunits from vector pGEX6P1 as polycistronic construct, in Escherichia coli strain Rosetta-2 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant subunts in a complex containing RNASEH2B residues 2-226, full-length RNASEH2C, and RNASEH2A with catalytic site mutations D34A and D169A, X-ray diffraction structure determination and analysis at 4.1-4.4 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A177T | naturally occuring mutation in subunit RNASEH2B, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS. The mutation disrupts the interface between a RNASEH2B alpha-helix and the RNASEH2C kinked helix | Homo sapiens |
D169A | active site residue mutation in subunit RNASEH2A | Homo sapiens |
D34A | active site residue mutation in subunit RNASEH2A | Homo sapiens |
K143I | naturally occuring mutation in subunit RNASEH2C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
P138L | naturally occuring mutation in subunit RNASEH2C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
P151S | naturally occuring mutation in subunit RNASEH2C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
P76L | naturally occuring mutation in subunit RNASEH2C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
R291H | naturally occuring mutation in subunit RNASEH2A, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
R69W | naturally occuring mutation in subunit RNASEH2C, the mutation is involved in auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate hydrolysis by RNase H2 follows Michaelis-Menten kinetics | Homo sapiens | |
0.000027 | - |
D14R1D3:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.000028 | - |
D14R1D3:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.00003 | - |
D14R1D3:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.000143 | - |
RNA18:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.000144 | - |
RNA18:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.000158 | - |
RNA18:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the eukaryotic RNase H2 heterotrimeric complex recognizes RNA/DNA hybrids and 5'RNA-DNA3'/DNA junction hybrids as substrates with similar efficiency | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75792 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D14R1D3:DNA18 + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the eukaryotic RNase H2 heterotrimeric complex recognizes RNA/DNA hybrids and 5'RNA-DNA3'/DNA junction hybrids as substrates with similar efficiency | Homo sapiens | ? | - |
? | |
RNA18:DNA18 + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | arrangement of subunits to form an enzymatically active complex, structure of the heterotrimeric RNase H2 complex, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H2 | - |
Homo sapiens |
RNase H2 | - |
Homo sapiens |
RNASEH2A | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.158 | - |
D14R1D3:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.35 | - |
D14R1D3:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.4 | - |
D14R1D3:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.983 | - |
RNA18:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
2.1 | - |
RNA18:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens | |
3.48 | - |
RNA18:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations in each of the three RNase H2 subunits are implicated in a human auto-inflammatory disorder, Aicardi-Goutieres syndrome, AGS | Homo sapiens |
additional information | the RNASEH2A C-terminus is a eukaryotic adaptation for binding the two accessory subunits, with residues within it required for enzymatic activity. This C-terminal extension interacts with the RNASEH2C C terminus and both are necessary to form a stable, enzymatically active heterotrimer | Homo sapiens |
physiological function | ribonuclease H2 is the major nuclear enzyme involved in the degradation of RNA/DNA hybrids and removal of ribonucleotides misincorporated in genomic DNA | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
D14R1D3:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
6.67 | - |
RNA18:DNA18 | mutant R291H, pH 8.0, temperature not specified in the publication | Homo sapiens | |
11.67 | - |
D14R1D3:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens | |
13.33 | - |
RNA18:DNA18 | mutant K143I, pH 8.0, temperature not specified in the publication | Homo sapiens | |
15 | - |
D14R1D3:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens | |
25 | - |
RNA18:DNA18 | wild-type enzyme, pH 8.0, temperature not specified in the publication | Homo sapiens |