Literature summary for 3.1.26.4 extracted from

Reiling, S.A.; Homma, K.; Asojo, O.A.
Purification and crystallization of RNase HIII from Staphylococcus aureus (2011), Acta Crystallogr. Sect. F, 67, 79-82.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of N-terminally His-tagged RNase HIII in Escherichia coli strain BL21 CodonPlus (DE3) RIPL	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme Sa-RNase HIII, sitting drop vapour diffusion method, 0.004 ml of 3.2 mg/ml protein in PBS containing 137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate dibasic, 2 mM potassium phosphate monobasic, pH 7.4, is mixed with 0.002 ml of precipitation solution containing 0.17 M ammonium sulfate, 25.5% w/v PEG 8000, 15% v/v glycerol, and 0.085 M sodium cacodylate pH 6.5, X-ray diffraction structure determination and analysis at 2.59-2.70 A resolution	Staphylococcus aureus

Crystallization (Comment)

Organism

purified recombinant enzyme Sa-RNase HIII, sitting drop vapour diffusion method, 0.004 ml of 3.2 mg/ml protein in PBS containing 137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate dibasic, 2 mM potassium phosphate monobasic, pH 7.4, is mixed with 0.002 ml of precipitation solution containing 0.17 M ammonium sulfate, 25.5% w/v PEG 8000, 15% v/v glycerol, and 0.085 M sodium cacodylate pH 6.5, X-ray diffraction structure determination and analysis at 2.59-2.70 A resolution

Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	Staphylococcus aureus
Mn2+	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Staphylococcus aureus	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	?	-	?
additional information	Staphylococcus aureus MRSA252	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus MRSA252	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His-tagged RNase HIII from Escherichia coli strain BL21 CodonPlus (DE3) RIPL by nickel affinity chromatography, dialysis and cleavage of the His-tag by TEV protease	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	Staphylococcus aureus	?	-	?
additional information	RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+	Staphylococcus aureus MRSA252	?	-	?

Synonyms

Synonyms	Comment	Organism
More	RNase HIII-type ribonucleases are members of the RNase H group of endonucleases	Staphylococcus aureus
RNase HIII	-	Staphylococcus aureus

General Information

General Information	Comment	Organism
physiological function	RNase HIII-type ribonucleases are members of the RNase H group of endonucleases which hydrolyze RNA from RNA/DNA hybrids and are possibly be involved in DNA replication and repair	Staphylococcus aureus