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Literature summary for 3.1.26.4 extracted from

  • Kitamura, S.; Fujishima, K.; Sato, A.; Tsuchiya, D.; Tomita, M.; Kanai, A.
    Characterization of RNase HII substrate recognition using RNase HII-argonaute chimaeric enzymes from Pyrococcus furiosus (2010), Biochem. J., 426, 337-344.
    View publication on PubMed
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional structural model of RNase HII complexed with its substrate, suggests that the amino acids D110, R113 and F114 are located in the region that discriminates DNA from RNA in the non-substrate strand of RNA-RNA Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
A115E partially enhanced nuclease activity for the RNA-DNA duplex substrate Pyrococcus furiosus
F114E reduced nuclease activity respectively for the RNA-DNA duplex substrate Pyrococcus furiosus
R113E partially reduced nuclease activity respectively for the RNA-DNA duplex substrate Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 RNA-DNA duplex mutant F114E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.00012
-
 RNA-DNA duplex wild-type, 50°C, presence of Mg2+ Pyrococcus furiosus
0.00012
-
 RNA-DNA duplex wild-type, 50°C, presence of Mn2+ Pyrococcus furiosus
0.00014
-
 RNA-DNA duplex mutant A115E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.0002
-
 RNA-DNA duplex mutant R113E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.0013
-
 RNA-RNA duplex wild-type, 50°C, presence of Mn2+ Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ wild-type digests RNA-RNA duplexes in presence of Mn2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U036
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA duplex + H2O
-
 Pyrococcus furiosus ?
-
 ?
RNA-RNA duplex + H2O reaction occurs in presence of Mn2+-ions. Residues D110, R113 and F114 are responsible for the activity, the residues are located in the region that discriminates DNA from RNA in the non-substrate strand of the duplexes Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
 RNA-RNA duplex wild-type, 50°C, presence of Mn2+ Pyrococcus furiosus
0.0068
-
 RNA-DNA duplex mutant F114E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.11
-
 RNA-DNA duplex mutant R113E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.188
-
 RNA-DNA duplex wild-type, 50°C, presence of Mn2+ Pyrococcus furiosus
0.255
-
 RNA-DNA duplex mutant A115E, 50°C, presence of Mn2+ Pyrococcus furiosus
0.283
-
 RNA-DNA duplex wild-type, 50°C, presence of Mg2+ Pyrococcus furiosus